Marinkova Dessislava, Bivolarska Maria, Ahtapodov Lyubomir, Yotova Lyubov, Mateva Rosa, Velinov Tzvetan
Department of Biotechnology, University of Chemical Technology and Metallurgy, 8 Kl. Ohridski Boulevard, 1756 Sofia, Bulgaria.
Colloids Surf B Biointerfaces. 2008 Sep 1;65(2):276-80. doi: 10.1016/j.colsurfb.2008.04.014. Epub 2008 May 4.
The attachment of Artrobacter oxydans 1,388 on a newly synthesized biodegradable copolymer of poly-(hexanlactam)-co-block-poly-(delta-valerolactone) is investigated by optical, microscopic and biochemical methods. The potentials of surface plasmon microscopy and imaging ellipsometry for detecting microorganisms when Al films are used to excite plasmons is assessed by comparing images obtained by these methods with dark field microscopy pictures. The experimental results demonstrate that in this case imaging ellipsometry and plasmon microscopy in transmission are promising methods and can be used in optical sensors for monitoring cell adhesion.
通过光学、显微镜和生化方法研究了氧化节杆菌1,388在新合成的聚（己内酰胺）-共-嵌段-聚（δ-戊内酯）生物可降解共聚物上的附着情况。通过将这些方法获得的图像与暗场显微镜图片进行比较，评估了使用铝膜激发等离子体时表面等离子体显微镜和成像椭偏仪检测微生物的潜力。实验结果表明，在这种情况下，成像椭偏仪和透射式等离子体显微镜是很有前景的方法，可用于光学传感器监测细胞粘附。
