Hyun Soonsil, Lee Eun Hye, Park Jihye, Yu Jaehoon
Department of Chemistry & Education, Seoul National University, San 56-1 Shinlim-dong, Kwanak-ku, Seoul 151-742, Republic of Korea.
Bioorg Med Chem Lett. 2008 Jul 15;18(14):4011-4. doi: 10.1016/j.bmcl.2008.06.003. Epub 2008 Jun 6.
An effort has generated peptides from a phage-displayed library that selectively bind to the sulfated carbohydrates HSO3-LeA and HSO3-LeX. Even though more than six phaged peptides were identified by using the biopanning procedure, only one synthesized peptide displayed a consistently high binding affinity and specificity against the cognate HSO3-LeA. This dimeric, tentacle type peptide has a low micromolar affinity against the cognate sugar, which is even more specific than an antibody (Table 2(b)). Thus, it suggests that tentacle type peptides can be used as alternatives to antibodies to bind to aberrant cell-surface carbohydrates that are either the causes or results of carbohydrate-indicating disease states.
人们已从噬菌体展示文库中筛选出能选择性结合硫酸化碳水化合物HSO3-LeA和HSO3-LeX的肽段。尽管通过生物淘选程序鉴定出了六种以上的噬菌体肽，但只有一种合成肽对同源HSO3-LeA表现出始终如一的高结合亲和力和特异性。这种二聚体触手型肽对同源糖具有低微摩尔亲和力，甚至比抗体更具特异性（表2(b)）。因此，这表明触手型肽可作为抗体的替代品，用于结合作为碳水化合物指示疾病状态的原因或结果的异常细胞表面碳水化合物。
