生长抑素对离体灌注大鼠胰腺中葡萄糖诱导的胰岛素释放的抑制作用。环磷酸腺苷、胰高血糖素和格列本脲的作用。
Inhibition of the glucose induced insulin release by somatostatin in the isolated perfused rat pancreas. Action of cyclic AMP, glucagon and glibenclamide.
作者信息
Osorio J, Campillo J E, de los Arcos J G, Pagés I, Rodríguez E, Marasco M, Melani F
出版信息
Rev Esp Fisiol. 1976 Sep;32(3):211-6.
Insulin release in the perfused isolated rat pancreas was measured after stimulation with 16.5 mM glucose with and without somatostatin (cycle form, 100 ng/ml) in the medium. A complete blockage of the typical biphasic pattern of insulin release ocurred with somatostatin in the medium. Such blockage was abolished when cAMP (2.5 mM) and a 0.5 ml solution of glucagon (1 mg/ml) were continuously perfused for 20-minute periods and for 30-second periods correspondently. It did not take place when glibenclamide (HB-419) was perfused for a 20-minute period at a rate of 10 mug/ml. The results suggest that the adenylcyclase dependent mechanisms of glucose-induced insulin release are involved in the inhibition of the glucose-induced insulin secretion by somatostatin.
在灌注的离体大鼠胰腺中，在培养基中添加和不添加生长抑素（环状形式，100 ng/ml）的情况下，用16.5 mM葡萄糖刺激后测量胰岛素释放。培养基中存在生长抑素时，胰岛素释放的典型双相模式完全被阻断。当持续灌注20分钟的cAMP（2.5 mM）和30秒的胰高血糖素溶液（1 mg/ml，0.5 ml）时，这种阻断被消除。以10 μg/ml的速率灌注20分钟的格列本脲（HB - 419）时，阻断未发生。结果表明，生长抑素对葡萄糖诱导的胰岛素分泌的抑制作用涉及葡萄糖诱导的胰岛素释放的腺苷酸环化酶依赖性机制。
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