Li Q Y, Chi Y Y, Liu S Q
Department of Urology and Transplantation, First Affiliated Hospital, Binzhou Medical University, Binzhou City, Shandong Province, China. Road, Binzhou City, Shandong Province 256603, China.
Immunopharmacol Immunotoxicol. 2008;30(2):365-81. doi: 10.1080/08923970801949174.
To probe into the mechanism of immunosuppression of FTY720, the authors study the cell cycle arrest effects of large-dose FTY720 on lymphocytes in mouse skin transplantation models and the expression of cell cycle-related factors in cell cycle regulation system in mouse skin allograft models using flow cytometry, Immunohistochemical staining, and reverse transcriptase-polymerase chain reaction (RT-PCR). FTY720 could prolong survival days of graft in mouse skin transplantation models obviously (p < 0.01). In thymus gland and lymph node, compared with the control group, cell percentage in G0-G1 increases and cell percentage in G2-M and S decreases in the treated group (p < 0.01). Expression of P16 increases and expression of cyclin D1 decreases in the treated group (p < 0.05). In thymus gland, it is shown by semiquantitative RT-PCR that the quantity of mRNA of P16 increases in the treated group (p < 0.01), and the quantity of mRNA of cyclin D1 decreases in the treated group (p < 0.01). FTY720 is a kind of effective immunosuppressant. FTY720 could induce cell cycle arrest in thymus gland and lymph node and change the expression of protein and the transcription of mRNA of cell cycle-related factor.
为探讨FTY720免疫抑制的机制，作者采用流式细胞术、免疫组织化学染色及逆转录聚合酶链反应（RT-PCR），研究大剂量FTY720对小鼠皮肤移植模型中淋巴细胞的细胞周期阻滞作用以及小鼠皮肤同种异体移植模型中细胞周期调节系统中细胞周期相关因子的表达。FTY720可显著延长小鼠皮肤移植模型中移植物的存活天数（p<0.01）。在胸腺和淋巴结中，与对照组相比，治疗组G0-G1期细胞百分比增加，G2-M期和S期细胞百分比降低（p<0.01）。治疗组P16表达增加，细胞周期蛋白D1表达降低（p<0.05）。在胸腺中，半定量RT-PCR显示治疗组P16的mRNA量增加（p<0.01），细胞周期蛋白D1的mRNA量降低（p<0.01）。FTY720是一种有效的免疫抑制剂。FTY720可诱导胸腺和淋巴结中的细胞周期阻滞，并改变细胞周期相关因子的蛋白表达和mRNA转录。
