Biochemical properties of ricin in immature castor seed.

作者信息

Chakravartula Srinivas V S, Guttarla Nagaraj

机构信息

Natural Products and Medicinal Chemistry Laboratory, Island Vitamins - Venus Pharmaceuticals Inc., Farmingdale, New York 11735, USA.

出版信息

Nat Prod Res. 2008 May 10;22(7):600-6. doi: 10.1080/14786410701602593.

DOI:10.1080/14786410701602593

PMID:18569697

Abstract

The biochemical properties of ricin at different stages of seed i.e. from immature to mature seed were studied. Hemagglutination, SDS-PAGE and UV-spectrometry studies showed total absence of RCA protein in the immature seed. Interestingly, ricin extract on SDS-PAGE showed only one protein band with a molecular weight of 29,000 dalton corresponding to the molecular weight of A chain of ricin. Our results have shown that at immature seed level only the toxic moiety of ricin (A chain) is being synthesized first and gradually the RCA and B chain of ricin.

摘要