Smith Carr J, Vournakis John N, Demcheva Marina, Fischer Thomas H
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Microsc Res Tech. 2008 Oct;71(10):721-9. doi: 10.1002/jemt.20612.
The design of devices for surface (topical) hemostasis has been based on maximizing activation of platelets and accelerating coagulation pathways. The studies reported herein examine another aspect of blood contact with topical hemostasis materials, i.e., surface binding of red blood cells (RBCs) and related alterations in RBC morphology. Whole blood was allowed to contact poly-N-acetyl glucosamine (pGlcNAc) containing materials: pGlcNAc nanofibers with parallel polymer alignment (beta-pGlcNAc), chitin, and chitosan. The effect on RBC morphology and function via contact with the artificial surfaces on the cell's morphology was examined with scanning and transmission electron microscopy (TEM). beta-pGlcNAc was found to densely bind RBCs and induce a stomatocytic-like morphology. Chitin and chitosan also bound RBCs, but with approximately 10-fold lower levels and with less distinct general morphologies. beta-pGlcNAc is thus unique in the nature of its interaction with RBCs. These studies indicate that the differential ability of various materials to bind and alter the morphology of RBCs at the artificial surface interface with blood is an important consideration in the design of devices for surface hemostasis.
用于表面（局部）止血的装置设计一直基于使血小板活化最大化并加速凝血途径。本文报道的研究探讨了血液与局部止血材料接触的另一个方面，即红细胞（RBC）的表面结合以及RBC形态的相关改变。使全血与含聚-N-乙酰葡糖胺（pGlcNAc）的材料接触：具有平行聚合物排列的pGlcNAc纳米纤维（β-pGlcNAc）、几丁质和壳聚糖。通过扫描电子显微镜和透射电子显微镜（TEM）检查了与人工表面接触对RBC形态和功能以及细胞形态的影响。发现β-pGlcNAc能紧密结合RBC并诱导出类似口形细胞的形态。几丁质和壳聚糖也能结合RBC，但结合水平约低10倍，且总体形态不太明显。因此，β-pGlcNAc与RBC相互作用的性质是独特的。这些研究表明，各种材料在与血液的人工表面界面处结合并改变RBC形态的不同能力是设计表面止血装置时的一个重要考虑因素。
