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PRG-1与21U-RNAs相互作用，形成秀丽隐杆线虫生育力所需的piRNA复合物。

PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans.

作者信息

Batista Pedro J, Ruby J Graham, Claycomb Julie M, Chiang Rosaria, Fahlgren Noah, Kasschau Kristin D, Chaves Daniel A, Gu Weifeng, Vasale Jessica J, Duan Shenghua, Conte Darryl, Luo Shujun, Schroth Gary P, Carrington James C, Bartel David P, Mello Craig C

机构信息

Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.

出版信息

Mol Cell. 2008 Jul 11;31(1):67-78. doi: 10.1016/j.molcel.2008.06.002. Epub 2008 Jun 19.

DOI:10.1016/j.molcel.2008.06.002
PMID:18571452
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2570341/
Abstract

In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-RNA loci share a conserved upstream sequence motif, the mature 21U-RNAs are not conserved and, with few exceptions, fail to exhibit complementarity or evidence for direct regulation of other expressed sequences. Our findings demonstrate that 21U-RNAs are the piRNAs of C. elegans and link this class of small RNAs and their associated Piwi Argonaute to the maintenance of temperature-dependent fertility.

摘要

在多细胞动物中，与Piwi相关的AGO蛋白与种系维持以及一类在种系中富集的小RNA（称为piRNA）有关。在此，我们表明，一类丰富的21个核苷酸的小RNA（21U-RNA）在秀丽隐杆线虫种系中表达，与秀丽隐杆线虫Piwi家族成员PRG-1相互作用，并依赖PRG-1的活性进行积累。PRG-1蛋白在整个发育过程中表达，并定位于称为P颗粒的类核仁结构。尽管21U-RNA基因座共享一个保守的上游序列基序，但成熟的21U-RNA并不保守，并且除了少数例外，未能表现出互补性或直接调控其他表达序列的证据。我们的研究结果表明，21U-RNA是秀丽隐杆线虫的piRNA，并将这类小RNA及其相关的Piwi AGO与温度依赖性生育力的维持联系起来。

相似文献

[1]
PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans.

Mol Cell. 2008-7-11

[2]
A C. elegans Piwi, PRG-1, regulates 21U-RNAs during spermatogenesis.

Curr Biol. 2008-6-24

[3]
C. elegans piRNAs mediate the genome-wide surveillance of germline transcripts.

Cell. 2012-6-25

[4]
Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline.

Mol Cell. 2008-7-11

[5]
The influences of PRG-1 on the expression of small RNAs and mRNAs.

BMC Genomics. 2014-4-30

[6]
PID-1 is a novel factor that operates during 21U-RNA biogenesis in Caenorhabditis elegans.

Genes Dev. 2014-4-1

[7]
piRNAs initiate an epigenetic memory of nonself RNA in the C. elegans germline.

Cell. 2012-6-25

[8]
PRDE-1 is a nuclear factor essential for the biogenesis of Ruby motif-dependent piRNAs in C. elegans.

Genes Dev. 2014-4-1

[9]
The C. elegans CSR-1 argonaute pathway counteracts epigenetic silencing to promote germline gene expression.

Dev Cell. 2013-12-23

[10]
Differential impact of the HEN1 homolog HENN-1 on 21U and 26G RNAs in the germline of Caenorhabditis elegans.

PLoS Genet. 2012-7-19

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[2]
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[3]
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[4]
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[5]
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[6]
WAGO-1 is a sexually dimorphic Argonaute protein required for proper germ granule structure and gametogenesis.

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[7]
Dynamic Birth and Death of Argonaute Gene Family Functional Repertoire Across Caenorhabditis Nematodes.

Genome Biol Evol. 2025-5-30

[8]
Dynamic control of Argonautes by a rapidly evolving immunological switch.

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[9]
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[10]
A nuclear pore-anchored condensate enables germ granule organization and transgenerational epigenetic inheritance.

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本文引用的文献

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Nat Rev Mol Cell Biol. 2008-1

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WormBook. 2005-7-28

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Development. 2008-1

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RNA. 2007-9

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MicroRNA targeting specificity in mammals: determinants beyond seed pairing.

Mol Cell. 2007-7-6

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Genes Dev. 2007-7-1

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Curr Biol. 2007-7-17

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Science. 2007-4-20

[9]
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Science. 2007-5-4

[10]
A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish.

Cell. 2007-4-6

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