HEN1的小鼠同源物是一种潜在的与Piwi相互作用RNA的甲基化酶。
The mouse homolog of HEN1 is a potential methylase for Piwi-interacting RNAs.
作者信息
Kirino Yohei, Mourelatos Zissimos
出版信息
RNA. 2007 Sep;13(9):1397-401. doi: 10.1261/rna.659307. Epub 2007 Jul 24.
Abstract
Piwi-interacting RNAs (piRNAs) are a novel class of small regulatory RNAs that are expressed specifically and abundantly in germ cells. Mammalian piRNAs are 26-31 nucleotides in length and bind to Piwi proteins, but their function and biogenesis remain elusive. We previously showed that mammalian piRNAs are 2'-O-methylated at their 3' termini. The biosynthetic mechanism and function of this modification is unknown. Here, we report that the mouse homolog (mHEN1) of HEN1, a plant microRNA (miRNA) 2'-O-methyltransferase, is expressed specifically in testis and methylates 3' termini of piRNAs in vitro. These findings provide insight into the biogenesis of piRNAs.
摘要
Piwi相互作用RNA（piRNA）是一类新型的小调节RNA，在生殖细胞中特异性且大量表达。哺乳动物的piRNA长度为26 - 31个核苷酸，并与Piwi蛋白结合，但其功能和生物合成机制仍不清楚。我们之前表明，哺乳动物的piRNA在其3'末端发生2'-O-甲基化。这种修饰的生物合成机制和功能尚不清楚。在这里，我们报道植物微小RNA（miRNA）2'-O-甲基转移酶HEN1的小鼠同源物（mHEN1）在睾丸中特异性表达，并在体外使piRNA的3'末端甲基化。这些发现为piRNA的生物合成提供了见解。
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本文引用的文献
1
Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi- interacting RNAs at their 3' ends.Pimet是HEN1在果蝇中的同源物，介导与Piwi相互作用的RNA在其3'端的2'-O-甲基化。
Genes Dev. 2007 Jul 1;21(13):1603-8. doi: 10.1101/gad.1563607.
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The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC.果蝇RNA甲基转移酶DmHen1可修饰生殖系piRNA和RISC中的单链siRNA。
Curr Biol. 2007 Jul 17;17(14):1265-72. doi: 10.1016/j.cub.2007.06.030. Epub 2007 Jun 28.
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A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish.Piwi蛋白和piRNA在斑马鱼生殖细胞维持及转座子沉默中的作用
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