[超活化豚鼠精子运动学参数分析]
[Analysis of the kinematic parameters of hyperactivated Guinea pig spermatozoa].
作者信息
Xu Lu, Huang Jing-Yan, Cui Qun-Wei, Wang Gen-Lin
机构信息
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
出版信息
Zhonghua Nan Ke Xue. 2008 May;14(5):387-90.
OBJECTIVE
To evaluate the role of sperm kinematic parameters in the hyperactivation of Guinea pig spermatozoa, and to confirm the index of their hyperactivated motility.
METHODS
Computer-aided sperm analysis (CASA) was used to describe the kinesis parameters of the Guinea pig spermatozoa incubated for 1, 3, 5 and 7 hours.
RESULTS
The curvilinear velocity, average path velocity and amplitude of lateral head displacement were increased with time and reached the peak at 5 hours, while the straight linear velocity, linearity, straightness and beat cross frequency were gradually decreased with time and hit the bottom at 5 hours.
CONCLUSION
The sperm movement pattern changes greatly before hyperactivation during the capacitation of Guinea pig spermatozoa.
目的
评估精子运动学参数在豚鼠精子超激活中的作用，并确定其超激活运动的指标。
方法
采用计算机辅助精子分析（CASA）描述豚鼠精子在孵育1、3、5和7小时后的运动参数。
结果
曲线速度、平均路径速度和头部侧摆幅度随时间增加，并在5小时达到峰值，而直线速度、线性度、直线性和鞭打交叉频率随时间逐渐降低，并在5小时达到最低点。
结论
豚鼠精子获能过程中，超激活前精子运动模式变化很大。
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