Wang Feng, Chen Lin, Mao Zhen-Biao, Shao Jian-Guo, Tan Chang, Huang Wei-Da
Department of Clinical Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
Oncol Rep. 2008 Jul;20(1):135-9.
RNA interference (RNAi) is an evolutionarily conserved process of gene silencing in multiple organisms, which has become a powerful tool for investigating gene function by reverse genetics. Herein, we constructed a short hairpin RNA (shRNA) lentiviral expression vector targeting a proliferation-inducing ligand (APRIL) gene in the CFPAC-1 cell (a type of cell strain of human pancreatic cancer) in order to observe the inhibitory effect of APRIL gene's shRNA on the growth of the CFPAC-1 cell in vitro and in vivo. The results showed that lentivirus-mediated RNAi effectively inhibited the expression of APRIL mRNA and protein in CFPAC-1 cells. Moreover, it can inhibit the growth of pancreatic cancer cells in vitro and in vivo. Our study indicates that lentivirus-mediated gene therapy is an attractive strategy in the treatment of pancreatic cancer and justifies the use of lentivirus in cancer gene therapy studies.
RNA干扰（RNAi）是多种生物体中一种进化上保守的基因沉默过程，它已成为通过反向遗传学研究基因功能的强大工具。在此，我们构建了一种靶向人胰腺癌细胞系CFPAC-1中增殖诱导配体（APRIL）基因的短发夹RNA（shRNA）慢病毒表达载体，以观察APRIL基因的shRNA在体外和体内对CFPAC-1细胞生长的抑制作用。结果表明，慢病毒介导的RNAi有效抑制了CFPAC-1细胞中APRIL mRNA和蛋白的表达。此外，它可以在体外和体内抑制胰腺癌细胞的生长。我们的研究表明，慢病毒介导的基因治疗是治疗胰腺癌的一种有吸引力的策略，证明了慢病毒在癌症基因治疗研究中的应用价值。
