• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

抗玉米赤霉烯酮功能性单链可变区片段抗体（scFv）在毕赤酵母中的表达

Expression of functional single-chain variable domain fragment antibody (scFv) against mycotoxin zearalenone in Pichia pastoris.

作者信息

Chang Hyun-Joo, Choi Sung-Wook, Chun Hyang Sook

机构信息

Korea Food Research Institute, 516, Backhyun, Bundang-gu, Sungnam, Kyonggi-do 463-746, South Korea.

出版信息

Biotechnol Lett. 2008 Oct;30(10):1801-6. doi: 10.1007/s10529-008-9770-x. Epub 2008 Jun 25.

DOI:10.1007/s10529-008-9770-x
PMID:18575809
Abstract

A synthetic gene coding for single-chain variable domain fragment antibody against mycotoxin zearalenone (scFv-ZEN) has been designed, constructed and expressed in Pichia pastoris. The native scFv-ZEN sequence was optimized to Pichia preference codon usage. The expression level of codon-optimized scFv-ZEN was slightly higher than that of native scFv-ZEN, and its maximum yield reached 328 mg total protein/l in flask culture. The binding activities of two selected clones to ZEN using surface plasmon resonance analysis were comparable or better than that of monoclonal antibody. Our results demonstrate the potential of soluble scFv-ZEN for developing a rapid and affordable immunoassay for detection of ZEN in food and feedstuff.

摘要

已设计、构建并在毕赤酵母中表达了一种编码抗玉米赤霉烯酮霉菌毒素单链可变区片段抗体（scFv-ZEN）的合成基因。将天然scFv-ZEN序列优化为毕赤酵母偏好的密码子使用方式。密码子优化后的scFv-ZEN的表达水平略高于天然scFv-ZEN，其在摇瓶培养中的最大产量达到328 mg总蛋白/升。使用表面等离子体共振分析，两个选定克隆与玉米赤霉烯酮的结合活性与单克隆抗体相当或更好。我们的结果证明了可溶性scFv-ZEN在开发用于检测食品和饲料中玉米赤霉烯酮的快速且经济实惠的免疫测定方法方面的潜力。

相似文献

1
Expression of functional single-chain variable domain fragment antibody (scFv) against mycotoxin zearalenone in Pichia pastoris.抗玉米赤霉烯酮功能性单链可变区片段抗体（scFv）在毕赤酵母中的表达
Biotechnol Lett. 2008 Oct;30(10):1801-6. doi: 10.1007/s10529-008-9770-x. Epub 2008 Jun 25.
2
Production of functionalized single-chain Fv antibody fragments binding to the ED-B domain of the B-isoform of fibronectin in Pichia pastoris.在毕赤酵母中产生与纤连蛋白B同工型的ED-B结构域结合的功能化单链Fv抗体片段。
Protein Expr Purif. 2001 Feb;21(1):156-64. doi: 10.1006/prep.2000.1362.
3
Codon optimization, expression, and characterization of an internalizing anti-ErbB2 single-chain antibody in Pichia pastoris.在毕赤酵母中对一种内化抗ErbB2单链抗体进行密码子优化、表达及特性鉴定
Protein Expr Purif. 2006 May;47(1):249-57. doi: 10.1016/j.pep.2005.11.014. Epub 2005 Dec 13.
4
High-level expression of a functional humanized single-chain variable fragment antibody against CD25 in Pichia pastoris.在毕赤酵母中高效表达针对CD25的功能性人源化单链可变片段抗体。
Appl Microbiol Biotechnol. 2008 Nov;81(1):33-41. doi: 10.1007/s00253-008-1568-1. Epub 2008 Aug 13.
5
Expression of an anti-CD33 single-chain antibody by Pichia pastoris.毕赤酵母表达抗CD33单链抗体
J Immunol Methods. 2005 Oct 30;305(2):135-51. doi: 10.1016/j.jim.2005.04.005. Epub 2005 Jun 16.
6
[Expression of anti-HIV envelope glycoprotein scFv in Pichia pastoris and its biologic activities analysis].[抗HIV包膜糖蛋白单链抗体在毕赤酵母中的表达及其生物学活性分析]
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Dec;23(12):1144-6.
7
Cloning, expression, and characterization of single-chain variable fragment antibody against mycotoxin deoxynivalenol in recombinant Escherichia coli.重组大肠杆菌中抗真菌毒素脱氧雪腐镰刀菌烯醇单链可变片段抗体的克隆、表达及鉴定
Protein Expr Purif. 2004 May;35(1):84-92. doi: 10.1016/j.pep.2003.12.008.
8
Construction of single chain variable fragment (ScFv) and BiscFv-alkaline phosphatase fusion protein for detection of Bacillus anthracis.用于检测炭疽芽孢杆菌的单链可变片段（ScFv）和双特异性单链抗体-碱性磷酸酶融合蛋白的构建
Anal Chem. 2006 Feb 15;78(4):997-1004. doi: 10.1021/ac0512352.
9
High-level expression of a functional humanized anti-CTLA4 single-chain variable fragment antibody in Pichia pastoris.一种功能性人源化抗CTLA4单链可变片段抗体在毕赤酵母中的高水平表达。
Appl Microbiol Biotechnol. 2009 Feb;82(1):41-8. doi: 10.1007/s00253-008-1744-3. Epub 2008 Oct 21.
10
Construction of multiform scFv antibodies using linker peptide.使用连接肽构建多种形式的单链抗体片段（scFv）抗体
J Genet Genomics. 2008 May;35(5):313-6. doi: 10.1016/S1673-8527(08)60045-4.

引用本文的文献

1
Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review.基于新兴生物识别分子的真菌毒素快速检测技术的最新进展：综述。
Toxins (Basel). 2022 Jan 20;14(2):73. doi: 10.3390/toxins14020073.
2
A new zearalenone biodegradation strategy using non-pathogenic Rhodococcus pyridinivorans K408 strain.利用非致病性吡啶红球菌 K408 菌株的新型玉米赤霉烯酮生物降解策略。
PLoS One. 2012;7(9):e43608. doi: 10.1371/journal.pone.0043608. Epub 2012 Sep 25.
3
Functional expression of recombinant anti-BNP scFv in methylotrophic yeast Pichia pastoris and application as a recognition molecule in electrochemical sensors.
在甲醇营养型酵母巴斯德毕赤酵母中表达重组抗脑利钠肽单链抗体及其作为电化学传感器识别分子的应用。
World J Microbiol Biotechnol. 2012 Mar;28(3):1027-34. doi: 10.1007/s11274-011-0901-5. Epub 2011 Sep 25.
4
Biochemical and biophysical characterization of humanized IgG1 produced in Pichia pastoris.毕赤酵母表达的人源化 IgG1 的生化和生物物理特性分析。
MAbs. 2011 Sep-Oct;3(5):453-60. doi: 10.4161/mabs.3.5.16891. Epub 2011 Sep 1.
5
Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments.运用实验设计优化毕赤酵母表达抗角蛋白 8 单链 Fv TS1-218。
Microb Cell Fact. 2011 May 16;10:34. doi: 10.1186/1475-2859-10-34.