Yoo Seung Sik, Park Sunghoon, Lee Eun Yeol
Department of Chemical Engineering, Pusan National University, Busan 609-735, South Korea.
Biotechnol Lett. 2008 Oct;30(10):1807-10. doi: 10.1007/s10529-008-9762-x. Epub 2008 Jun 25.
The reaction medium was optimized to accomplish epoxide hydrolase-catalyzed, batch enantioselective hydrolysis of racemic styrene oxide at high initial substrate concentrations. The recombinant Pichia pastoris containing the epoxide hydrolase gene of Rhodotorula glutinis was used as the biocatalyst. Enantiopure (S)-styrene oxide with 98% ee was obtained with 41% yield (maximum yield = 50%) from 1.8 M racemic styrene oxide at pH 8.0, 4 degrees C in the presence of 40% (v/v) Tween 20 and 5% (v/v) glycerol.
对反应介质进行了优化，以实现环氧水解酶催化的外消旋环氧苯乙烯在高初始底物浓度下的间歇对映选择性水解。含有粘红酵母环氧水解酶基因的重组毕赤酵母用作生物催化剂。在pH 8.0、4℃、存在40%（v/v）吐温20和5%（v/v）甘油的条件下，以1.8 M外消旋环氧苯乙烯为原料，获得了对映体纯的（S）-环氧苯乙烯，对映体过量值为98%，产率为41%（最大产率 = 50%）。
