一种改进的从牛肝中纯化亚硫酸盐氧化酶的方法。

An improved purification procedure for sulfite oxidase from bovine liver.

作者信息

Cabré F, Cascante M, Canela E I

机构信息

Department of Biochemistry and Physiology, Faculty of Chemistry, University of Barcelona, Catalonia, Spain.

出版信息

Prep Biochem. 1991;21(1):53-61. doi: 10.1080/10826069108021515.

DOI:10.1080/10826069108021515

PMID:1857684

Abstract

Sulfite oxidase (Sulfite:O2 oxidoreductase, EC 1.8.3.1) has been purified 2,440-fold from bovine liver. The procedure developed was used to isolate the enzyme from 1,000 g of liver and permitted the rapid isolation of enzyme with a very high specific activity (40,405 mU/mg). The enzyme preparations obtained have been characterized by electrophoretic and spectrophotometric analysis and the molecular mass and the Stokes radius of the enzyme have been determined.

摘要

亚硫酸盐氧化酶（亚硫酸盐：氧气氧化还原酶，EC 1.8.3.1）已从牛肝中纯化了2440倍。所开发的方法用于从1000克肝脏中分离该酶，并能快速分离出具有非常高比活性（40,405 mU/mg）的酶。通过电泳和分光光度分析对所获得的酶制剂进行了表征，并测定了该酶的分子量和斯托克斯半径。