Mergny J L, Slama-Schwok A, Montenay-Garestier T, Rougée M, Hélène C
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, Paris, France.
Photochem Photobiol. 1991 Apr;53(4):555-8. doi: 10.1111/j.1751-1097.1991.tb03670.x.
Dimethyldiazaperopyrenium is one of the largest known DNA intercalators. Fluorescence energy transfer occurred between dimethyldiazaperopyrenium (donor) and ethidium (acceptor) when these dyes were bound to a double-stranded polynucleotide such as poly d(A-T). The addition of increasing amounts of ethidium bromide led to a marked shortening of the fluorescence lifetime of the donor, whereas the excited state of the acceptor was progressively populated via energy transfer from the donor. Critical Förster distance between these two chromophores was calculated to be 3.8 nm. The observed transfer efficiency was lower than that calculated on the basis of this critical distance and a statistical distribution of bound drugs. These results are discussed taking into account the conformational change induced by intercalation of dimethyldiazaperopyrenium in the double-stranded polynucleotide.
二甲基二氮杂芘鎓是已知最大的DNA嵌入剂之一。当这些染料与双链多核苷酸如聚d(A-T)结合时，二甲基二氮杂芘鎓（供体）和溴化乙锭（受体）之间会发生荧光能量转移。加入越来越多的溴化乙锭会导致供体荧光寿命显著缩短，而受体的激发态则通过从供体的能量转移逐渐形成。计算得出这两个发色团之间的临界福斯特距离为3.8纳米。观察到的转移效率低于根据该临界距离和结合药物的统计分布计算得出的效率。结合二甲基二氮杂芘鎓嵌入双链多核苷酸引起的构象变化对这些结果进行了讨论。
