Establishment of the comet assay in the freshwater snail Biomphalaria glabrata (Say, 1818).
作者信息
Grazeffe Vanessa Siqueira, Tallarico Lenita de Freitas, Pinheiro Alessandro de Sá, Kawano Toshie, Suzuki Miriam Fussae, Okazaki Kayo, Pereira Carlos Alberto de Bragança, Nakano Eliana
机构信息
Laboratório de Parasitologia/Malacologia, Instituto Butantan, Avenida Vital Brasil, 1500, CEP 05503-900, São Paulo, Brazil.
出版信息
Mutat Res. 2008 Jun 30;654(1):58-63. doi: 10.1016/j.mrgentox.2008.05.007. Epub 2008 May 21.
The single cell gel electrophoresis or the comet assay was established in the freshwater snail Biomphalaria glabrata. For detecting DNA damage in circulating hemocytes, adult snails were irradiated with single doses of 2.5, 5, 10 and 20 Gy of (60)Co gamma radiation. Genotoxic effect of ionizing radiation was detected at all doses as a dose-related increase in DNA migration. Comet assay in B. glabrata demonstrated to be a simple, fast and reliable tool in the evaluation of genotoxic effects of environmental mutagens.
Zotero 插件