Morad Mostafa Y, El-Sayed Heba, Elhenawy Ahmed A, Korany Shereen M, Aloufi Abeer S, Ibrahim Amina M
Zoology and Entomology Department, Faculty of Science, Helwan University, Helwan 11795, Egypt.
Botany and Microbiology Department, Faculty of Science, Helwan University, Helwan 11795, Egypt.
J Fungi (Basel). 2022 Mar 4;8(3):262. doi: 10.3390/jof8030262.
Schistosomiasis is a tropical disease with socioeconomic problems. The goal of this study was to determine the influence of myco-synthesized nano-selenium (SeNPs) as a molluscicide on snails, with the goal of reducing disease spread via non-toxic routes. In this study, culture filtrate metabolites were used as a reductant for selenium ions to form nano-selenium. The SeNPs were characterized via UV-Vis spectrophotometer, Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), and X-ray diffraction (XRD). Myco-synthesized SeNPs had a significant molluscicidal effect on snails after 96 h of exposure at a concentration of 5.96 mg/L. SeNPs also had miracidicidal and cercaricidal properties against . Some alterations were observed in the hemocytes of snails exposed to SeNPs, including the formation of pseudopodia and an increasing number of granules. Furthermore, lipid peroxide, nitric oxide (NO), malondialdehyde (MDA), and glutathione s-transferase (GST) increased significantly in a dose-dependent manner, while superoxide dismutase (SOD) decreased. The comet assay revealed that myco-synthesized SeNPs could cause breaks in the DNA levels. In silico study revealed that SeNPs had promising antioxidant properties. In conclusion, myco-synthesized SeNPs have the potential to be used as molluscicides and larvicides.
血吸虫病是一种伴有社会经济问题的热带疾病。本研究的目的是确定作为杀螺剂的真菌合成纳米硒（SeNPs）对蜗牛的影响，目标是通过无毒途径减少疾病传播。在本研究中，培养滤液代谢物被用作硒离子的还原剂以形成纳米硒。通过紫外可见分光光度计、傅里叶变换红外（FT-IR）光谱、透射电子显微镜（TEM）、动态光散射（DLS）和X射线衍射（XRD）对SeNPs进行了表征。在浓度为5.96 mg/L的条件下暴露96小时后，真菌合成的SeNPs对蜗牛具有显著的杀螺作用。SeNPs对[具体寄生虫名称缺失]也具有杀蚴和杀尾蚴特性。在暴露于SeNPs的蜗牛血细胞中观察到一些变化，包括伪足的形成和颗粒数量的增加。此外，脂质过氧化物、一氧化氮（NO）、丙二醛（MDA）和谷胱甘肽S-转移酶（GST）呈剂量依赖性显著增加，而超氧化物歧化酶（SOD）减少。彗星试验表明，真菌合成的SeNPs可导致DNA水平断裂。计算机模拟研究表明，SeNPs具有良好的抗氧化性能。总之，真菌合成的SeNPs有潜力用作杀螺剂和杀幼虫剂。
