黑腹果蝇中P转座因子的细胞型调控：阻遏多肽还是piRNA？
Cytotype regulation of P transposable elements in Drosophila melanogaster: repressor polypeptides or piRNAs?
作者信息
Jensen Philip A, Stuart Jeremy R, Goodpaster Michael P, Goodman Joseph W, Simmons Michael J
机构信息
Department of Genetics, Cell Biology, and Development, University of Minnesota, St. Paul, Minnesota 55108-1095, USA.
出版信息
Genetics. 2008 Aug;179(4):1785-93. doi: 10.1534/genetics.108.087072. Epub 2008 Jun 24.
Abstract
The telomeric P elements TP5 and TP6 are associated with the P cytotype, a maternally inherited condition that represses P-element-induced hybrid dysgenesis in the Drosophila germ line. To see if cytotype repression by TP5 and TP6 might be mediated by the polypeptides they could encode, hobo transgenes carrying these elements were tested for expression of mRNA in the female germ line and for repression of hybrid dysgenesis. The TP5 and TP6 transgenes expressed more germ-line mRNA than the native telomeric P elements, but they were decidedly inferior to the native elements in their ability to repress hybrid dysgenesis. These paradoxical results are inconsistent with the repressor polypeptide model of cytotype. An alternative model based on the destruction of P transposase mRNA by Piwi-interacting (pi) RNAs was supported by finding reduced P mRNA levels in flies that carried the native telomeric P elements, which are inserted in a known major piRNA locus.
摘要
端粒P元件TP5和TP6与P细胞型相关，P细胞型是一种母系遗传的状态，可抑制果蝇生殖系中P元件诱导的杂种不育。为了探究TP5和TP6对细胞型的抑制作用是否可能由它们编码的多肽介导，对携带这些元件的hobo转基因进行了检测，以观察其在雌性生殖系中的mRNA表达以及对杂种不育的抑制情况。TP5和TP6转基因在雌性生殖系中表达的mRNA比天然端粒P元件更多，但在抑制杂种不育的能力方面，它们明显不如天然元件。这些矛盾的结果与细胞型的阻遏多肽模型不一致。基于Piwi相互作用（pi）RNA对P转座酶mRNA的破坏作用的另一种模型得到了支持，因为在携带插入已知主要piRNA基因座的天然端粒P元件的果蝇中，发现P mRNA水平降低。
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