Hudelist Gernot, Huber Ambros, Knoefler Martin, Haider Sandra, Kolbus Andrea, Czerwenka Klaus, Helmy Samir, Kubista Ernst, Singer Christian F
Division of Special Gynaecology, Department of Obstetrics and Gynaecology, Medical University of Vienna, Vienna, Austria.
Reprod Sci. 2008 Jul;15(6):543-51. doi: 10.1177/1933719108316907.
Luteinizing hormone (LH) and human chorionic gonadotropin (HCG) target their receptor in gonadal and nongonadal cells to stimulate steroidogenesis and cell growth. The aim of the present study was to investigate the expression of HCG/LH-R in endometriosis to elucidate a possible impact of LH and HCG on this disease.
Analysis of HCG/LH-R protein expression in 23 paired samples of ectopic and eutopic tissue of cycling women with endometriosis and in endometrial samples from 22 healthy controls was conducted via immunofluorescence. HCG and HCG/LH-R gene expression in endometriotic lesions was confirmed by reverse-transcriptase polymerase chain reaction.
In endometriotic implants, epithelial HCG/LH-R was found in 12/23 samples. No significant differences in HCG/LH-R levels were observed when compared with glands of uterine endometrium from the same patients or healthy controls. Messenger RNA transcripts for HCG were detected in all 12 samples, whereas HCG/LH-R mRNAs were observed in 10 of the 12 endometriotic lesions investigated.
Although HCG/LH-R was not found to be selectively upregulated in endometriosis, the mere presence of HCG/LH-R in endometriotic tissue may suggest sensitivity of endometriosis to HCG and LH that target HCG/LH-R.
促黄体生成素（LH）和人绒毛膜促性腺激素（HCG）作用于性腺和非性腺细胞中的受体，以刺激类固醇生成和细胞生长。本研究的目的是调查子宫内膜异位症中HCG/LH-R的表达，以阐明LH和HCG对该疾病可能产生的影响。
通过免疫荧光分析23例患有子宫内膜异位症的育龄妇女异位和在位组织的配对样本以及22例健康对照者的子宫内膜样本中HCG/LH-R蛋白的表达。通过逆转录聚合酶链反应确认子宫内膜异位症病变中HCG和HCG/LH-R基因的表达。
在子宫内膜异位植入物中，12/23的样本中发现上皮细胞有HCG/LH-R。与同一患者的子宫内膜腺体或健康对照相比，未观察到HCG/LH-R水平有显著差异。在所有12个样本中均检测到HCG的信使核糖核酸转录本，而在所研究的12个子宫内膜异位病变中的10个中观察到了HCG/LH-R的信使核糖核酸。
虽然未发现子宫内膜异位症中HCG/LH-R有选择性上调，但子宫内膜异位组织中单纯存在HCG/LH-R可能提示子宫内膜异位症对作用于HCG/LH-R的HCG和LH敏感。
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