Edjème-Aké Angèle, Garnotel Roselyne, Vallée-Polneau Sandrine, Anglés-Cano Eduardo, Monnet Dagui, Gillery Philippe
Laboratory of Biochemistry, UFR Pharmaceutical and Biological Sciences, Abidjan, Côte d'Ivoire.
Clin Biochem. 2008 Sep;41(13):1039-43. doi: 10.1016/j.clinbiochem.2008.05.014. Epub 2008 Jun 12.
To determine the relationship between apo(a) size and Lp(a) concentration in healthy Ivorian subjects.
Serum Lp(a) was measured by immunonephelometry and electroimmunodiffusion, and apo(a) size determined by immunoblotting.
Both assay methods provided comparable information. Lp(a) concentrations showed a non-Gaussian distribution and skewed towards higher values. Apo(a) isoform size distribution exhibited three frequency peaks at 18, 22 and 25 kringles. Single and double apo(a) isoforms were detected in 36% and 64% of subjects, respectively. Lp(a) values were higher in subjects with double isoforms, the major isoform being of lower size. An inverse correlation between apo(a) size and Lp(a) concentration was found, apo(a) size accounting for more than 30% of Lp(a) concentration in "single-band" group, whereas being weaker in "double-band" group. Low size isoforms were associated with higher Lp(a) concentrations, high size isoforms with higher variability.
Lp(a) concentrations were inversely correlated to apo(a) size. This study has shown the relationship between apo(a) size and Lp(a), the influence of apo(a) size varying according to the phenotype. Apo(a) "double isoform" phenotype confers elevated levels to Lp(a) in healthy Ivorian subject.
确定健康科特迪瓦受试者中载脂蛋白(a)大小与脂蛋白(a)[Lp(a)]浓度之间的关系。
采用免疫比浊法和电免疫扩散法测定血清Lp(a)，通过免疫印迹法测定载脂蛋白(a)大小。
两种检测方法提供了可比的信息。Lp(a)浓度呈非高斯分布且偏向较高值。载脂蛋白(a)异构体大小分布在18、22和25个kringle处呈现三个频率峰值。分别在36%和64%的受试者中检测到单载脂蛋白(a)异构体和双载脂蛋白(a)异构体。双异构体受试者的Lp(a)值较高，主要异构体的大小较小。发现载脂蛋白(a)大小与Lp(a)浓度呈负相关，在“单带”组中载脂蛋白(a)大小占Lp(a)浓度的30%以上，而在“双带”组中相关性较弱。小尺寸异构体与较高的Lp(a)浓度相关，大尺寸异构体与较高的变异性相关。
Lp(a)浓度与载脂蛋白(a)大小呈负相关。本研究显示了载脂蛋白(a)大小与Lp(a)之间的关系，载脂蛋白(a)大小的影响因表型而异。在健康科特迪瓦受试者中，载脂蛋白(a)“双异构体”表型使Lp(a)水平升高。
