Veremeenko K N, Varetskaia T V, Kizim A I, Svital'skaia L A
Vopr Med Khim. 1991 Jan-Feb;37(1):44-6.
Simple procedure was developed for production of fibrinogen concentrate from small amounts of human autogenous blood using precipitation with polyethylene glycol and ammonium sulfate. Maximal yield of fibrinogen was obtained using polyethylene glycol 2,000 and 6,000 daltons at concentrations 7% and 4.5%, respectively. The fibrinogen preparations included fibronectin, inhibitors of proteinases, factor XIII of blood coagulation, plasminogen. Sigma-Aminocapronic acid and contrical should be added during bloodletting as well as into blood plasma before isolation of fibrinogen to avoid possible fibrinogen proteolysis.
开发了一种简单的方法，通过使用聚乙二醇和硫酸铵沉淀，从小量人体自体血液中生产纤维蛋白原浓缩物。分别使用浓度为7%的聚乙二醇2000和浓度为4.5%的聚乙二醇6000可获得最大纤维蛋白原产量。纤维蛋白原制剂包括纤连蛋白、蛋白酶抑制剂、凝血因子 XIII、纤溶酶原。在放血期间以及在分离纤维蛋白原之前向血浆中添加6-氨基己酸和抑肽酶，以避免可能的纤维蛋白原蛋白水解。
