Miyano Naoki, Inoue Yuuki, Teramura Yuji, Fujii Keisuke, Tsumori Fujio, Iwata Hiroo, Kotera Hidetoshi
Department of Microengineering, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
Lab Chip. 2008 Jul;8(7):1104-9. doi: 10.1039/b718655j. Epub 2008 May 15.
In the diffusional phase transformation of two-phase alloys, the new phase precipitates form the matrix phase at specific temperatures, followed by the formation of a mixed microstructure comprising the precipitate and the matrix. It has been found that by specific chemical-etching treatment, the precipitate in Fe-25Cr-6Ni alloy projects substantially and clusters at the surface. The configuration of the precipitate has an extremely high aspect ratio: it is several microns in width and several tens of microns in length (known as micron-spiked). This study targets the development of a gene transfer device with a micro-spike produced based on the self-organization phenomenon of the Fe-25Cr-6Ni alloy. With this spike-projected device, we tried to efficiently transfer plasmid DNA into adherent cells by electric pulse-triggered gene transfer using a plasmid-loaded electrode (electroporation-based reverse transfection). The spiked structure was applied to a substrate of the device to allow efficient gene transfer into adherent cells, although the general substrate was flat and had a smooth surface. The results suggest that this unique spike-projected device has potential applications in gene transfer devices for the analysis of the human genome in the post-genome period.
在两相合金的扩散型相变过程中，新相在特定温度下从基体相中析出，随后形成由析出相和基体组成的混合微观结构。研究发现，通过特定的化学蚀刻处理，Fe-25Cr-6Ni合金中的析出相在表面显著突出并聚集。析出相的形态具有极高的长径比：宽度为几微米，长度为几十微米（称为微米尖刺状）。本研究旨在基于Fe-25Cr-6Ni合金的自组织现象开发一种具有微尖刺的基因转移装置。利用这种带有尖刺突出结构的装置，我们尝试通过使用加载质粒的电极进行电脉冲触发的基因转移（基于电穿孔的反向转染），将质粒DNA高效地转移到贴壁细胞中。尽管一般的装置基底是平坦且表面光滑的，但这种尖刺结构被应用于装置的基底上，以实现向贴壁细胞的高效基因转移。结果表明，这种独特的带有尖刺突出结构的装置在基因组后期人类基因组分析的基因转移装置中具有潜在应用价值。
