Lau Adrian Y, Lee Luke P, Chan James W
Applied Physics and Biophysics Division, Lawrence Livermore National Laboratory, P.O. Box 808, L-211, Livermore, CA 94550, USA.
Lab Chip. 2008 Jul;8(7):1116-20. doi: 10.1039/b803598a. Epub 2008 May 28.
We report on integrated optofluidic Raman-activated cell sorting (RACS) platforms that combine multichannel microfluidic devices and laser tweezers Raman spectroscopy (LTRS) for delivery, identification, and simultaneous sorting of individual cells. The system allows label-free cell identification based on Raman spectroscopy and automated continuous cell sorting. Two optofluidic designs using hydrodynamic focusing and pinch-flow fractionation are evaluated based on their sorting design and flow velocity effect on the laser trapping efficiency at different laser power levels. A proof-of-principle demonstration of the integrated optofluidic LTRS system for the identification and sorting of two leukemia cell lines is presented. This functional prototype lays the foundation for the development of a label-free cell sorting platform based on intrinsic Raman markers for automated sampling and sorting of a large number of individual cells in solution.
我们报道了集成光流拉曼激活细胞分选（RACS）平台，该平台结合了多通道微流控装置和激光镊子拉曼光谱（LTRS），用于单个细胞的输送、识别和同时分选。该系统允许基于拉曼光谱进行无标记细胞识别以及自动连续细胞分选。基于流体动力聚焦和夹流分级分离的两种光流设计，根据其分选设计以及在不同激光功率水平下流速对激光捕获效率的影响进行了评估。展示了用于识别和分选两种白血病细胞系的集成光流LTRS系统的原理验证。这个功能原型为基于固有拉曼标记物的无标记细胞分选平台的开发奠定了基础，该平台可用于对溶液中的大量单个细胞进行自动采样和分选。
