Hossain M M, Do D D
Department of Chemical Engineering, University of Queensland, St. Lucia, Queensland 4067, Australia.
Biotechnol Bioeng. 1988 May;31(7):730-6. doi: 10.1002/bit.260310716.
Experiments have been carried out for immobilizing enzyme-catalase and amyloglucosidase in controlled-pore glass particles of two different pore sizes. The experimental results have been analyzed, initial-stage analysis for the rate parameters of immobilization reactions and long-time analysis for determining the evolution of the immobilization process. These investigations suggest that the overall process of immobilization is controlled by the restricted diffusion of enzymes into the pores of the support. As a result, immobilized enzyme (IME) can penetrate only up to a certain distance into the support. The penetration depth of IME for the support-enzyme system mentioned have been evaluated from the experimental bulk enzyme concentration data in a batch recirculation reactor.
已经进行了将过氧化氢酶和糖化酶固定在两种不同孔径的可控孔玻璃颗粒中的实验。对实验结果进行了分析，对固定化反应的速率参数进行了初始阶段分析，并对确定固定化过程的演变进行了长期分析。这些研究表明，固定化的整个过程受酶向载体孔中受限扩散的控制。因此，固定化酶（IME）只能渗透到载体中一定距离。已经根据间歇循环反应器中的实验总体酶浓度数据评估了上述载体 - 酶系统中IME的渗透深度。
