Acker G, Wartenberg K
Zentralbl Bakteriol Orig A. 1976 Aug;235(4):439-52.
The fine structure of isolated lipopolysaccharides (LPS) from the rough and smooth form of an Yersinia enterocolitica strain (Ye 75 R/Ye 75 S) and from smooth forms of Salmonella typhimurium (S 1010) and Escherichia coli (Essen) were examined electron-microscopically by negative staining. Partial denaturation of LPS in Tris-buffer with acid and/or polymyxin B treatment revealed a common structure of strandlike LPS. Electron-microscopically, LPS-strands were found to consist of two identical sub-strands which form a double helix. High resolution electron microscopy permitted the identification of a total of four longitudinal fibrils (diameter approximately 20 A); therefore, each sub-strand consists of two longitudinal fibrils. These results were correlated with those obtained with positive staining procedure and chemical fixation technique. The development of the "double-track"-profile of the outer membrane was interpreted to result from the projection of the helical longitudinal fibrils into the image plane.
通过负染色法对小肠结肠炎耶尔森菌菌株（Ye 75 R/Ye 75 S）粗糙型和光滑型以及鼠伤寒沙门氏菌（S 1010）和大肠杆菌（埃森株）光滑型中分离出的脂多糖（LPS）的精细结构进行了电子显微镜检查。用酸和/或多粘菌素B处理Tris缓冲液中的LPS使其部分变性，揭示了链状LPS的共同结构。在电子显微镜下，发现LPS链由两条相同的子链组成，形成双螺旋结构。高分辨率电子显微镜能够识别出总共四条纵向纤维（直径约20埃）；因此，每条子链由两条纵向纤维组成。这些结果与用正染色程序和化学固定技术获得的结果相关。外膜“双轨”轮廓的形成被解释为螺旋纵向纤维投影到图像平面的结果。
