牙髓感染中特定呼吸道病原体的鉴定
Identification of selected respiratory pathogens in endodontic infections.
作者信息
Nandakumar Renu, Whiting Justin, Fouad Ashraf F
机构信息
Department of Endodontics, Prosthodontics and Operative Dentistry, Dental School, University of Maryland, Baltimore 21201, USA.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Jul;106(1):145-51. doi: 10.1016/j.tripleo.2008.01.036.
OBJECTIVE
To determine whether endodontic infections could harbor common etiologic agents of respiratory infections such as Streptococcus pneumoniae and Chlamydia pneumoniae.
STUDY DESIGN
Specimens were aseptically obtained from 40 patients with endodontic infections. For the detection of C. pneumoniae, single-step 16S rRNA-based polymerase chain reaction (PCR) and nested PCR targeting aromatic amino acid hydroxylase were used. For the identification of S. pneumoniae, primers targeting 16S rRNA gene and autolysin (lytA) were used.
RESULTS
Of 21 patient samples tested with the 16S rRNA-based PCR for S. pneumoniae, positive amplification was observed in all except 3 specimens. However, sequencing and phylogenetic analysis revealed that the product belonged to other bacterial phylotypes. The lytA-based PCR for S. pneumoniae and both PCR assays for C. pneumoniae failed to detect these organisms in all of the specimens tested.
CONCLUSIONS
Streptococcus pneumoniae and C. pneumoniae were not present in endodontic infections. PCR primers with less stringent specificity will inaccurately identify respiratory pathogens.
目的
确定牙髓感染是否携带有呼吸道感染的常见病原体，如肺炎链球菌和肺炎衣原体。
研究设计
从40例牙髓感染患者中无菌获取标本。对于肺炎衣原体的检测，使用基于16S rRNA的单步聚合酶链反应（PCR）和靶向芳香族氨基酸羟化酶的巢式PCR。对于肺炎链球菌的鉴定，使用靶向16S rRNA基因和自溶素（lytA）的引物。
结果
在用基于16S rRNA的PCR检测肺炎链球菌的21份患者样本中，除3份标本外，所有样本均观察到阳性扩增。然而，测序和系统发育分析表明，产物属于其他细菌系统型。用于肺炎链球菌的基于lytA的PCR以及用于肺炎衣原体的两种PCR检测方法在所有测试标本中均未检测到这些病原体。
结论
牙髓感染中不存在肺炎链球菌和肺炎衣原体。特异性较低的PCR引物会不准确地鉴定呼吸道病原体。
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