Blumöhr T, Klöcking R, Sprössig M, Wöckel W
Acta Biol Med Ger. 1976;35(1):103-9.
Basing on the experimental infection of adult mice with coxsackie virus A9 reported by Lerner in 1965 and the successful cultivation of the virus from the hearts of experimental animals, a model for studying virus myocarditis is proposed. By a combination of factors favouring the infection (age of the animals above 1 year, physical load prior to infection by repeated swimming) a high virus re-isolation rate was achieved. Cultivation of the virus from the mouse heart was possible on the 3rd day p.i. in 81%, on the 6th day in 71%, on the 9th day in 31%, and on the 12th day in 12% of the cases. In 66% of the animals towards the end of the period of observation a significant increase in few cases is the virus isolated on the 3rd and 6th day p.i. also from the skeletal muscles. The model can be used both for pathogenetic studies on myocardites elicited by cardiotropic viruses and for in vivo testing of antiviral substances.
基于1965年勒纳报道的成年小鼠柯萨奇病毒A9实验性感染以及从实验动物心脏成功培养出该病毒，提出了一种研究病毒性心肌炎的模型。通过综合有利于感染的因素（动物年龄超过1岁，感染前通过反复游泳进行体力负荷），实现了较高的病毒重新分离率。在感染后第3天，81%的病例能从小鼠心脏培养出病毒；第6天为71%；第9天为31%；第12天为12%。在66%的动物中，在观察期结束时，少数病例出现显著增加，在感染后第3天和第6天从骨骼肌中也分离出了病毒。该模型可用于对嗜心性病毒引起的心肌炎进行发病机制研究，也可用于抗病毒物质的体内测试。
