Rozee K R, Klassen G A, Ahmad-Raza A, Lee S H
Department of Microbiology, Victoria General Hospital, Halifax, Nova Scotia.
Can J Cardiol. 1992 Mar;8(2):145-8.
To develop a mouse model of coxsackievirus B3 (CVB3) myocarditis.
Preliminary studies have indicated that mice infected with CVB3 alone erratically responded with viral myocarditis. Prospective evaluation of the effect of cyclophosphamide at two time intervals resulted in consistency for the development of myocarditis.
Juvenile five- to nine-week-old male mice CD-1 type (Charles River Canada Limited).
Infection with coxsackie B3 enterovirus. Pretreatment with cyclophosphamide two days and 4 h before inoculation of 0.2 or 0.15 mg/g. Animals were killed seven, nine, 28 and 63 days post infection.
Following cyclophosphamide conditioning, a tissue response infection with CVB3 was uniformly observed. Virus, however, was infrequently recovered from the myocardium whereas myocarditis became chronic after 28 days.
Pretreatment of juvenile mice with cyclophosphamide results in a reproducible model of viral myocarditis with chronic changes in the myocardium.
建立柯萨奇病毒B3（CVB3）心肌炎小鼠模型。
初步研究表明，单独感染CVB3的小鼠对病毒性心肌炎的反应不稳定。对两个时间间隔使用环磷酰胺的效果进行前瞻性评估，结果显示心肌炎的发生具有一致性。
5至9周龄的幼年雄性CD-1型小鼠（加拿大查尔斯河有限公司）。
感染柯萨奇B3肠道病毒。在接种前2天和4小时用0.2或0.15mg/g的环磷酰胺进行预处理。感染后7天、9天、28天和63天处死动物。
经过环磷酰胺预处理后，均观察到CVB3感染后的组织反应。然而，很少从心肌中分离出病毒，而心肌炎在28天后变为慢性。
用环磷酰胺预处理幼年小鼠可建立可重复的病毒性心肌炎模型，心肌会出现慢性变化。
