一种用于测定硒代胱氨酸和硒代蛋氨酸的新型高效液相色谱-紫外/纳米二氧化钛-化学发光系统。
A novel HPLC-UV/nano-TiO2-chemiluminescence system for the determination of selenocystine and selenomethionine.
作者信息
Su Yingying, Chen He, Gao Ying, Li Xiaohong, Hou Xiandeng, Lv Yi
机构信息
Analytical & Testing Center, Sichuan University, Chengdu, Sichuan 610064, China.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jul 15;870(2):216-21. doi: 10.1016/j.jchromb.2008.06.012. Epub 2008 Jun 13.
Active oxygen species from the photocatalytic reaction in aqueous solution react with luminol to emit strong chemiluminescence (CL), and this can be inhibited by the UV decomposed-products of selenocystine (SeCys) or selenomethionine (SeMet). Based on this phenomenon, a novel hyphenated technique, HPLC-UV/nano-TiO(2)-CL, was established for the determination of SeCys and SeMet. The effects of pH, the UV irradiation time, the TiO(2) coated on the inner surface of the reaction tubing, and the Co(2+) catalyst concentration on the CL intensity and/or chromatographic resolution were systematically investigated. Under these optimized conditions, the inhibited CL intensity has a good linear relationship with the concentration of SeCys in the range of 0.04-10.6 microg mL(-1) or SeMet in the range of 0.05-12.4 microg mL(-1), with a limit of detection (S/N=3) of 6.4 microg L(-1) for SeCys or 12 microg L(-1) for SeMet. As an example, the method was preliminarily applied to the determination of the selenoamino acids in garlic and rabbit serum, with a recovery of 88-104%.
水溶液中光催化反应产生的活性氧物种与鲁米诺反应发出强烈的化学发光（CL），而这会被硒代胱氨酸（SeCys）或硒代蛋氨酸（SeMet）的紫外分解产物抑制。基于此现象，建立了一种用于测定SeCys和SeMet的新型联用技术，即HPLC-UV/纳米TiO₂-CL。系统研究了pH、紫外照射时间、反应管内表面涂覆的TiO₂以及Co²⁺催化剂浓度对CL强度和/或色谱分辨率的影响。在这些优化条件下，抑制的CL强度与浓度范围为0.04 - 10.6 μg mL⁻¹的SeCys或浓度范围为0.05 - 12.4 μg mL⁻¹的SeMet具有良好的线性关系，SeCys的检测限（S/N = 3）为6.4 μg L⁻¹，SeMet的检测限为12 μg L⁻¹。作为示例，该方法初步应用于大蒜和兔血清中硒代氨基酸的测定，回收率为88 - 104%。
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