Liang Lina, Mo Shumin, Zhang Ping, Cai Yaqi, Mou Shifen, Jiang Guibin, Wen Meijuan
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
J Chromatogr A. 2006 Jun 16;1118(1):139-43. doi: 10.1016/j.chroma.2006.03.113. Epub 2006 Apr 25.
A technique for the speciation of selenomethylcysteine (SeMeCys), selenocystine (SeCys), selenite [Se(IV)] and selenomethionine (SeMet) was established in this paper using high-performance anion-exchange chromatography coupled with atomic fluorescence spectrometry (HPAEC-AFS). Analytes were separated on an AminoPac PA10 column and then digested by on-line ultraviolet (UV) irradiation, which destroyed organic compound structure. Hydride generation was used as an available sample introduction technique for atomic fluorescence detection. The detection limits of four compounds were 1-5 microg/L (250 microL injection, 10 times of the baseline noise). The relative standard deviations (RSDs), calculated from seven consecutive injections of 100 microg/L standard mixtures, were from 2 to 4%. Selenious yeast tablet, which had been proposed as selenium supplement, and human urine collected from a volunteer were analyzed. Good spiked recoveries from 86 to 103% were obtained.
本文建立了一种利用高效阴离子交换色谱-原子荧光光谱联用技术（HPAEC-AFS）对硒代半胱氨酸（SeMeCys）、硒代胱氨酸（SeCys）、亚硒酸盐[Se(IV)]和硒代蛋氨酸（SeMet）进行形态分析的方法。分析物在AminoPac PA10柱上分离，然后通过在线紫外（UV）照射进行消解，破坏有机化合物结构。氢化物发生法用作原子荧光检测的有效进样技术。四种化合物的检测限为1-5μg/L（进样250μL，基线噪声的10倍）。由100μg/L标准混合物连续进样7次计算得到的相对标准偏差（RSD）为2%-4%。对已被提议作为硒补充剂的亚硒酸钠片和从一名志愿者收集的人尿进行了分析。加标回收率良好，为86%-103%。
