Tsuboi Yumi, Munemoto Haruko, Ishikawa Shizuma, Matsumoto Ken-ichi, Iguchi-Ariga Sanae M M, Ariga Hiroyoshi
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo, Japan.
FEBS Lett. 2008 Jul 23;582(17):2643-9. doi: 10.1016/j.febslet.2008.06.043. Epub 2008 Jun 27.
DJ-1 is secreted into the serum and plasma of patients with various diseases. In this study, DJ-1 was found to be secreted into culture media of various cells and the amount of wild-type DJ-1 secreted was two-fold greater than that of mutant DJ-1 of cysteine at 106 (C106). Furthermore, the oxidative status of more than 90% of the DJ-1 secreted from HeLa cells was SOH and SO2H forms of C106. A portion of DJ-1 in cells was localized in microdomains of the membrane. These findings suggest that DJ-1 is secreted through microdomains and that oxidation of DJ-1 at C106 facilitates the secretion.
DJ-1会分泌到患有各种疾病患者的血清和血浆中。在本研究中，发现DJ-1会分泌到各种细胞的培养基中，且野生型DJ-1的分泌量比106位半胱氨酸（C106）的突变型DJ-1高出两倍。此外，从HeLa细胞分泌的DJ-1中，超过90%的氧化状态为C106的SOH和SO2H形式。细胞中的一部分DJ-1定位于细胞膜的微结构域。这些发现表明，DJ-1是通过微结构域分泌的，并且C106处DJ-1的氧化促进了分泌。
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