Wang Lin, Wei Li, Miao Shuxian, Zhang Wei
Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Branch of National Clinical Research Center for Laboratory Medicine, Nanjing, China.
PeerJ. 2024 Jan 29;12:e16845. doi: 10.7717/peerj.16845. eCollection 2024.
DJ-1 is an oncoprotein secreted by cancer cells. However, the physiological and pathological significance of DJ-1 secretion is not clearly understood. This study investigated the clinical value of serum DJ-1 in lung adenocarcinoma (LUAD).
The study involved 224 LUAD patients, 110 patients with benign pulmonary disease and 100 healthy controls from the First Affiliated Hospital of Nanjing Medical University. We detected the expression of DJ-1 in lung cell lines . Meanwhile, serum concentrations of DJ-1, carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), and cytokeratin 19 fragment (CYFRA21-1) were measured. The diagnostic performance of LUAD was obtained using receiver operating characteristic (ROC) curves. Kaplan-Meier, univariate and multivariate Cox regression analyses were performed for progression-free survival (PFS).
DJ-1 was highly expressed in LUAD cell lines. Serum DJ-1 levels were significantly higher in the LUAD group compared to the benign pulmonary disease group (5.04 3.66 ng/mL, < 0.001) and healthy controls (5.04 3.51 ng/mL, < 0.001). DJ-1 levels were associated with gender ( = 0.002), smoking history ( = 0.042) and lymph node metastasis ( = 0.040). ROC curve analysis of DJ-1 revealed an area under the curve (AUC) of 0.758 (95% CI [0.714-0.803], < 0.001) with a sensitivity of 63.8% and specificity of 78.6% at a cutoff value of 4.62 ng/mL for the detection of LUAD. Univariate and multivariate analyses confirmed that the preoperative serum DJ-1 level, tumor stage and smoking history were independent prognostic factors of PFS.
Our study is the first to explore the clinical value of serum DJ-1 in LUAD comprehensively. Serum DJ-1 could be a potential diagnostic and prognostic biomarker for LUAD.
DJ-1是一种由癌细胞分泌的癌蛋白。然而，DJ-1分泌的生理和病理意义尚不清楚。本研究探讨血清DJ-1在肺腺癌（LUAD）中的临床价值。
本研究纳入了南京医科大学第一附属医院的224例LUAD患者、110例良性肺部疾病患者和100例健康对照者。我们检测了DJ-1在肺癌细胞系中的表达。同时，测定了血清中DJ-1、癌胚抗原（CEA）、神经元特异性烯醇化酶（NSE）和细胞角蛋白19片段（CYFRA21-1）的浓度。使用受试者工作特征（ROC）曲线获得LUAD的诊断性能。对无进展生存期（PFS）进行了Kaplan-Meier单因素和多因素Cox回归分析。
DJ-1在LUAD细胞系中高表达。LUAD组血清DJ-1水平显著高于良性肺部疾病组（5.04±3.66 ng/mL，P<0.001）和健康对照组（5.04±3.51 ng/mL，P<0.001）。DJ-1水平与性别（P=0.002）、吸烟史（P=0.042）和淋巴结转移（P=0.040）相关。DJ-1的ROC曲线分析显示曲线下面积（AUC）为0.758（95%CI[0.714-0.803]，P<0.001），在检测LUAD时，截断值为4.62 ng/mL时，灵敏度为63.8%，特异性为78.6%。单因素和多因素分析证实，术前血清DJ-1水平、肿瘤分期和吸烟史是PFS的独立预后因素。
我们的研究首次全面探讨了血清DJ-1在LUAD中的临床价值。血清DJ-1可能是LUAD的一种潜在诊断和预后生物标志物。
