固定化脂肪酶在亲水膜反应器中对甘油三酯的水解作用。

The hydrolysis of triglycerides by immobilized lipase in a hydrophilic membrane reactor.

作者信息

Pronk W, Kerkhof P J, Van Helden C, Riet K V

机构信息

Department of Food Science, Food and Bioengineering Group, Agricultural University Wageningen, De Dreijen 12, 6703 BC Wageningen, The Netherlands.

出版信息

Biotechnol Bioeng. 1988 Aug 5;32(4):512-8. doi: 10.1002/bit.260320414.

DOI:10.1002/bit.260320414

PMID:18587749

Abstract

In the present article a method is described to immobilize lipase from Candida rugosa on a hollow fiber membrane, and the use of such a system for the hydrolysis of lipids is reported. The membranes were ENKA hydrophilic Cuprophan-type hollow fibers, having a large specific surface area. The immobilized lipase exhibited a high stability: the half-life time was 43 days at a temperature of 30 degrees C. Furthermore, it is proved that kinetic studies can be carried out with this system, operated in a batch or continuous mode. The relation between conversion rate and degree of hydrolysis was determined. On this basis, a dynamic model of the process was developed that describes the relation between reaction conditions and the conversion rate.

摘要

在本文中，描述了一种将皱褶假丝酵母脂肪酶固定在中空纤维膜上的方法，并报道了使用该系统进行脂质水解的情况。这些膜是恩卡亲水性铜氨型中空纤维，具有较大的比表面积。固定化脂肪酶表现出高稳定性：在30℃温度下半衰期为43天。此外，证明了可以用该系统进行动力学研究，该系统可以分批或连续模式运行。确定了转化率与水解程度之间的关系。在此基础上，建立了一个描述反应条件与转化率之间关系的过程动态模型。