Ryhiner G, Petrozzi S, Dunn I J
Chemical Engineering Department, ETH, 8092 Zurich, Switzerland.
Biotechnol Bioeng. 1988 Aug 20;32(5):677-88. doi: 10.1002/bit.260320513.
A biofilm fluidized sand bed column reactor (14 L) has been operated in the three-phase mode on a soluble glucose-yeast hydrolysate substrate in which the biofilm-sand phase (1-2.5 L) was suspended by direct aeration of the bed. Within two weeks a tight biofilm was formed whose activity resulted in a 90% reduction, with loads of 10.7 kg TC/m(3)day. The residence time was 1 h. The biofilm remained intact during operation with high residence times (up to 23 h) over three weeks. Oxygen transfer coefficients varied with aeration rate and sand quantity between 0.02 and 0.04 s(-1) during non growth conditions; they decreased with increasing amounts of clean sand and were higher and relatively independent of the sand fraction with biofilm-covered sand. Aeration rates used in the 14 L reactor were 23-40 L/min (2.4-4.1 cm/s) and were sufficient to suspend 78-92% f the biofilm-covered sand. Clean sand was 50-75% suspended. Oxygen uptake rates varied between 15.4 and 23.1 mol/m(3) h.
一个生物膜流化床砂床柱式反应器（14升）以三相模式运行，以可溶性葡萄糖 - 酵母水解产物为底物，通过直接对床层曝气使生物膜 - 砂相（1 - 2.5升）保持悬浮状态。在两周内形成了紧密的生物膜，其活性使负荷为10.7千克总碳/立方米·天的情况下，去除率达到90%。停留时间为1小时。在长达三周的高停留时间（长达23小时）运行期间，生物膜保持完整。在非生长条件下，氧传递系数随曝气速率和砂量的变化在0.02至0.04秒⁻¹之间；随着清洁砂量的增加而降低，对于覆盖有生物膜的砂，氧传递系数较高且相对独立于砂的粒径。14升反应器中使用的曝气速率为23 - 40升/分钟（2.4 - 4.1厘米/秒），足以使78 - 92%的覆盖有生物膜的砂保持悬浮状态。清洁砂的悬浮率为50 - 75%。氧吸收率在15.4至23.1摩尔/立方米·小时之间变化。
