同型乙酸发酵过程中克利夫氏梭菌对乳酸的反应动力学及数学模型研究。

Kinetics and mathematical modeling of homoacetic fermentation of lactate by Clostridium formicoaceticum.

机构信息

Department of Chemical Engineering, The Ohio State University, Columbus, Ohio 43210, USA.

出版信息

Biotechnol Bioeng. 1988 Sep 5;32(6):797-802. doi: 10.1002/bit.260320611.

DOI:10.1002/bit.260320611

Abstract

Fermentation kinetics of Clostridium formicoaceticum grown on lactate at pH 7.0 and 35 degrees C was studied. Acetate was the only fermentation product and its production was growth associated. The growth of this bacterium was insensitive to the lactate concentrations studied, but was inhibited by acetic acid. A Monod-type expression with product inhibition similar to the noncompetitive inhibition of enzyme kinetics was used to model the batch fermentation. An integrated equation was developed and used to help estimating the kinetic parameters in the model. This mathematical model can be used to simulate the homoacetic fermentation of lactate by C. formicoaceticum at pH 7.0 and 35 degrees C.

摘要

在 pH 值为 7.0 和 35 摄氏度的条件下，研究了在乳酸上生长的产甲酸乙酸梭菌的发酵动力学。乙酸是唯一的发酵产物，其生成与生长相关。该细菌的生长对所研究的乳酸浓度不敏感，但受到乙酸的抑制。使用类似于酶动力学的非竞争性抑制的产物抑制的 Monod 型表达来对批式发酵进行建模。开发了一个综合方程，并用于帮助估计模型中的动力学参数。该数学模型可用于模拟 pH 值为 7.0 和 35 摄氏度时 C. formicoaceticum 对乳酸的同型乙酸发酵。