Mu Yang, Yang Hou-Yun, Wang Ya-Zhou, He Chuan-Shu, Zhao Quan-Bao, Wang Yi, Yu Han-Qing
Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China.
Sci Rep. 2014 Jun 10;4:5239. doi: 10.1038/srep05239.
Fermentative hydrogen production from wastes has many advantages compared to various chemical methods. Methodology for characterizing the hydrogen-producing activity of anaerobic mixed cultures is essential for monitoring reactor operation in fermentative hydrogen production, however there is lack of such kind of standardized methodologies. In the present study, a new index, i.e., the maximum specific hydrogen-producing activity (SHAm) of anaerobic mixed cultures, was proposed, and consequently a reliable and simple method, named SHAm test, was developed to determine it. Furthermore, the influences of various parameters on the SHAm value determination of anaerobic mixed cultures were evaluated. Additionally, this SHAm assay was tested for different types of substrates and bacterial inocula. Our results demonstrate that this novel SHAm assay was a rapid, accurate and simple methodology for determining the hydrogen-producing activity of anaerobic mixed cultures. Thus, application of this approach is beneficial to establishing a stable anaerobic hydrogen-producing system.
与各种化学方法相比，利用废物发酵产氢具有许多优势。表征厌氧混合培养物产氢活性的方法对于监测发酵产氢过程中的反应器运行至关重要，然而目前缺乏此类标准化方法。在本研究中，提出了一个新指标，即厌氧混合培养物的最大比产氢活性（SHAm），并由此开发了一种可靠且简单的方法——SHAm测试法来测定该指标。此外，评估了各种参数对厌氧混合培养物SHAm值测定的影响。另外，针对不同类型的底物和细菌接种物对该SHAm测定法进行了测试。我们的结果表明，这种新型的SHAm测定法是一种快速、准确且简单的测定厌氧混合培养物产氢活性的方法。因此，应用该方法有助于建立稳定的厌氧产氢系统。
