Mizumoto Y, Fujimura Y, Nishikawa K, Uchida M, Fukui H, Morii T, Narita N, Kurata Y
Department of the Second Internal Medicine, Nara Medical College, Osaka University Hospital, Japan.
Am J Hematol. 1991 Aug;37(4):274-6. doi: 10.1002/ajh.2830370413.
Acid treatment of normal washed platelets prepared by albumin density gradient centrifugation followed by formalin fixation effectively eliminates HLA class I antigens from the platelet surface, without altering the immunoreactivity of platelet membrane glycoproteins Ib and IIb/IIIa complex. Using these platelets, flow cytometric analysis was performed on a total of 20 sera from different patients with chronic ITP, and revealed an inverse linear correlation between the platelet count and the mean fluorescence intensity which represents a relative amount of platelet-bound IgG. These results suggest that acid-treated, formalin-fixed platelets can be used as specimens to detect anti-platelet antibodies to differentiate from those to HLA-class I antigens.
通过白蛋白密度梯度离心制备正常洗涤血小板，然后用福尔马林固定，经酸处理可有效去除血小板表面的HLA - I类抗原，而不改变血小板膜糖蛋白Ib和IIb/IIIa复合物的免疫反应性。使用这些血小板，对20例不同慢性免疫性血小板减少症（ITP）患者的血清进行了流式细胞术分析，结果显示血小板计数与代表血小板结合IgG相对量的平均荧光强度之间呈负线性相关。这些结果表明，经酸处理、福尔马林固定的血小板可作为检测抗血小板抗体的标本，以区分与HLA - I类抗原相关的抗体。
