Department of Chemical Engineering, University of Osaka Prefecture, 804 Mozu-Umemachi 4-cho, Sakai, Osaka 591, Japan.
Biotechnol Bioeng. 1989 Jul;34(3):369-79. doi: 10.1002/bit.260340310.
Enzymatic synthesis of glucose 6-phosphate from glucose and ATP catalyzed by glucokinase from B. stearothermophilus and enzymatic regeneration of ATP from ADP and acetyl phosphate catalyzed by acetatekinase from B. stearothermophilus were simultaneously performed in an Ultrafiltration hollow-fiber reactor of the multitubular heat-exchanger type. Experimental results of space-time yield, the conversion, and ATP recycle number were in good agreement with the theoretical predictions based on the simple analytical model developed in the preceding article. The best results for space-time yield and conversion were Y(s) = 1.97 mol/m(3) h and X = 92.8%, respectively, under the same conditions, and the best result for the ATP recycle number was N(R) = 2130 under conditions different from those above. However, Y(s) = 1.72 mol/m(3) h, X = 81.2%, and N(R) = 1620 were the results when the space-time yield, conversion, and recycle number were at the highest in combination under the same conditions. Results of long-term operation showed that the apparent remaining activity of the enzyme system was ca. 55% after continuous operation for 16 days, the decrease in the enzyme activity being faster than that expected from their half-life times determined individually in the homogeneous system.
嗜热脂肪芽孢杆菌的葡萄糖激酶催化葡萄糖和 ATP 合成葡萄糖 6-磷酸，以及乙酸激酶催化 ADP 和乙酰磷酸合成 ATP 的酶促反应在多管式热交换型中空纤维反应器中同时进行。时空产率、转化率和 ATP 循环数的实验结果与前文所建立的简单分析模型的理论预测吻合良好。在相同条件下，时空产率和转化率的最佳结果分别为 Y(s) = 1.97 mol/m(3) h 和 X = 92.8%，而在不同条件下，ATP 循环数的最佳结果为 N(R) = 2130。然而，当在相同条件下将时空产率、转化率和循环数组合至最高时，Y(s) = 1.72 mol/m(3) h、X = 81.2%和 N(R) = 1620 是最佳结果。长期运行结果表明，在连续运行 16 天后，酶体系的表观剩余活性约为 55%，酶活性的下降速度快于在均相体系中单独测定的半衰期所预期的下降速度。
