Li D H, Ganczarczyk J J
Department of Civil Engineering, University of Toronto, Toronto, Ontario, Canada.
Biotechnol Bioeng. 1990 Jan 5;35(1):57-65. doi: 10.1002/bit.260350109.
Relatively large activated sludge floes (larger than about 100 microm) were stabilized, using a histological tissue specimen preparation procedure, and then were sliced into sections of 3 to 6 microm thick. The study of these sections, after staining, revealed the internal structure of the activated sludge floes. No uniformity of this structure was found. The distribution of microorganisms and of extracellular polymers (EPs) in the floes varied randomly on the plane of the sections and along the dimension perpendicular to the plane, leaving large water channels and reservoirs in some of the floes. The lack of a characteristic size for the water gaps in the floes and a general self-similar appearance of the sections suggested that the activated sludge floes might be characterized by the fractal concept within a certain size limit. Direct observation of the interior of the floes indicated an abundant presence of extracellular polymers (EPs) in amorphous forms, surrounding microorganisms in most of the floes.
使用组织学组织标本制备程序使相对较大的活性污泥絮体（大于约100微米）稳定下来，然后将其切成3至6微米厚的切片。对这些切片进行染色后研究发现了活性污泥絮体的内部结构。未发现这种结构具有均匀性。微生物和细胞外聚合物（EPs）在絮体中的分布在切片平面上以及垂直于该平面的维度上随机变化，在一些絮体中留下了较大的水道和储水空间。絮体中水间隙缺乏特征尺寸以及切片总体上具有自相似外观表明，活性污泥絮体在一定尺寸范围内可能具有分形概念特征。对絮体内部的直接观察表明，在大多数絮体中，无定形形式的细胞外聚合物（EPs）大量存在，围绕着微生物。
