Barbour H M
Department of Clinical Chemistry, Western General Hospital, Edinburgh, UK.
Ann Clin Biochem. 1991 Mar;28 ( Pt 2):150-4. doi: 10.1177/000456329102800205.
A method for the simultaneous determination of sweat sodium and chloride was developed and evaluated using a Monarch 2000 centrifugal analyser with ion-selective electrodes. In order to measure sodium and chloride in diluted sweat samples and sweat paper eluates an analytical working range of below 10 mmol/L was required. This was achieved by modification of the Monarch ISE software parameters for urine electrolytes together with the use of calibrators within this range. Evaluation of the method has included linearity, recovery and precision studies and the correlation between ion-selective electrode and flame photometry for sweat sodium and between ion-selective electrode and a continuous flow method for sweat chloride. Of the patients studied so far, there has been good discrimination in sweat electrolytes between those with and without cystic fibrosis.
开发了一种使用配备离子选择电极的Monarch 2000离心分析仪同时测定汗液钠和氯的方法并进行了评估。为了测量稀释汗液样本和汗液纸洗脱液中的钠和氯，需要低于10 mmol/L的分析工作范围。这是通过修改用于尿液电解质的Monarch ISE软件参数并使用该范围内的校准物来实现的。该方法的评估包括线性、回收率和精密度研究，以及离子选择电极与汗液钠火焰光度法之间以及离子选择电极与汗液氯连续流动法之间的相关性。在迄今为止研究的患者中，患有和未患有囊性纤维化的患者在汗液电解质方面有很好的区分度。
