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荧光测量的标准化:选择合适标准的标准以及适用校准工具的定制方法。

Standardization of fluorescence measurements: criteria for the choice of suitable standards and approaches to fit-for-purpose calibration tools.

作者信息

Resch-Genger Ute, Hoffmann Katrin, Hoffmann Angelika

机构信息

Federal Institute for Materials Research and Testing (BAM), I.5 Bioanalytics, Richard-Willstaetter-Str. 11, 12489 Berlin-Adlershof, Germany.

出版信息

Ann N Y Acad Sci. 2008;1130:35-43. doi: 10.1196/annals.1430.018.

Abstract

This report summarizes problems associated with the comparability of measurements of photoluminescence and procedures for the characterization of relevant instruments, focusing on physical and chemical fluorescence standards. To provide recommendations on selecting and using such standards, we derive general and scope-specific requirements and quality criteria for suitable devices and materials and briefly address metrological requirements linked to the realization of comparable measurements. Special emphasis is dedicated to liquid and solid chromophore-based fluorescence standards developed or currently tested by us.

摘要

本报告总结了与光致发光测量的可比性以及相关仪器表征程序相关的问题,重点关注物理和化学荧光标准。为了就此类标准的选择和使用提供建议,我们推导了适用于合适设备和材料的一般及特定范围要求和质量标准,并简要阐述了与实现可比测量相关的计量要求。特别强调了我们开发或目前正在测试的基于液体和固体发色团的荧光标准。

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