溶剂分子毒性和微环境组成对节杆菌细胞中 Delta(1)脱氢酶活性的影响。

Effects of solvent molecular toxicity and microenvironment composition on the Delta(1) dehydrogenataon activity of Arthrobacter simplex cells.

机构信息

Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, 1000 Lisbon, Portugal.

出版信息

Biotechnol Bioeng. 1991 Jan 20;37(2):97-102. doi: 10.1002/bit.260370202.

Abstract

Solvent selection tests were carried out for the Delta(1) dehydrogenation of 6-alpha-methylhydrocortisone-21-acetate by Arthrobacter simplex cells in the presence of organic solvents. Solubility limits were determined for substrate and product in dry and water-saturated solvents and solvent mixtures. Molecular toxicity levels were estimated by measuring the dehydrogenation activity decay of freely suspended cells incubated in solvent-saturated aqueous media. Chloroform and n-decan-1-ol were the best choice of solvent, for both solubility and catalytic stability. High concentrations of water-soluble additives, such as monosodium glutamate, were found to greatly improve the retention of activity in chloroform-saturated media.

摘要

溶剂选择试验在有机溶剂存在的情况下，用 Simplex 细胞对 6-α-甲基氢化可的松-21-醋酸酯的 Δ(1)脱氢作用进行。在干燥和水饱和溶剂及溶剂混合物中，测定了底物和产物的溶解度极限。通过测量在溶剂饱和水介质中孵育的自由悬浮细胞的脱氢活性衰减，估算了分子毒性水平。氯仿和正癸-1-醇是最适合的溶剂，既具有良好的溶解性又具有催化稳定性。发现高浓度的水溶性添加剂，如谷氨酸单钠，可大大提高在氯仿饱和介质中保持活性的能力。