Kobayashi T, Yasuda A, Yamaguchi K, Kawauchi H, Kikuyama S
Department of Biology, School of Education, Waseda University, Tokyo, Japan.
Biochim Biophys Acta. 1991 Jul 12;1078(3):383-7. doi: 10.1016/0167-4838(91)90160-2.
The primary structure of growth hormone (GH) isolated from the adenohypophysis of the bullfrogs (Rana catesbeiana) was determined. The hormone was reduced, carboxymethylated and subsequently cleaved with cyanogen bromide. Intact bullfrog GH was also digested with lysyl endopeptidase and trypsin. The resulting fragments were separated by reverse-phase high-performance liquid chromatography and subjected to sequence analysis using an automated gas-liquid sequencer employing the Edman method. Bullfrog GH was found to consist of 190 amino acid residues. The amino acid sequence determined is in accord with that deduced from bullfrog GH cDNA by Pan and Chang (1988) except for nine residues at positions 43-48, 73, 80 and 87. Sequence comparisons revealed that bullfrog GH is more similar to tetrapod GHs (e.g., 69% homology with sea turtle GH, 66% with chicken GH and 61% with ovine GH) than to GHs of teleosts (e.g., 35% homology with chum salmon GH and 33% with bonito GH) except for eel (52% identity). Bullfrog GH and prolactin exhibit a sequence homology of 25%.
测定了从牛蛙（牛蛙属）腺垂体中分离出的生长激素（GH）的一级结构。该激素经还原、羧甲基化处理，随后用溴化氰裂解。完整的牛蛙GH还用赖氨酰内肽酶和胰蛋白酶进行了消化。所得片段通过反相高效液相色谱分离，并使用采用埃德曼法的自动气液测序仪进行序列分析。发现牛蛙GH由190个氨基酸残基组成。所确定的氨基酸序列与潘和张（1988年）从牛蛙GH cDNA推导的序列一致，只是在第43 - 48、73、80和87位有9个残基不同。序列比较显示，牛蛙GH与四足动物的GH（例如，与海龟GH的同源性为69%，与鸡GH的同源性为66%，与绵羊GH的同源性为61%）比与硬骨鱼的GH（例如，与大麻哈鱼GH的同源性为35%，与鲣鱼GH的同源性为33%）更为相似，鳗鱼除外（同一性为52%）。牛蛙GH和催乳素的序列同源性为25%。
