Baldridge G D, Fallon A M
Department of Entomology, University of Minnesota, St. Paul 55108.
Biochim Biophys Acta. 1991 Jul 23;1089(3):396-400. doi: 10.1016/0167-4781(91)90184-n.
We have sequenced an 18S ribosomal RNA gene from the mosquito, Aedes albopictus. Computer alignment of the 1950 nucleotide coding region (56% A + T) with 18S rRNA sequences from two insect and three vertebrate species revealed greater sequence divergence among the insects than among the vertebrates. Sequence alignments showed that variable region V4, which has been considered to be the most poorly conserved domain in the 18S rRNA gene, was better conserved among insects and vertebrates than was the V6 domain.
我们对来自白纹伊蚊的18S核糖体RNA基因进行了测序。将1950个核苷酸编码区(A+T含量为56%)与来自两种昆虫和三种脊椎动物的18S rRNA序列进行计算机比对,结果显示昆虫之间的序列差异大于脊椎动物之间的差异。序列比对表明,被认为是18S rRNA基因中保守性最差的结构域的可变区V4,在昆虫和脊椎动物中比V6结构域的保守性更好。