转化生长因子β亚型（TGF-β1、TGF-β2、TGF-β3）在喉癌中的信使核糖核酸表达
Transforming growth factor beta isoforms (TGF-beta1, TGF-beta2, TGF-beta3) messenger RNA expression in laryngeal cancer.
作者信息
Kapral Malgorzata, Strzalka Barbara, Kowalczyk Malgorzata, Jurzak Magdalena, Mazurek Urszula, Gierek Tatiana, Paluch Jaroslaw, Markowski Jaroslaw, Swiatkowska Longina, Weglarz Ludmila
机构信息
Department of Biochemistry, Medical University of Silesia, Sosnowiec, Poland.
出版信息
Am J Otolaryngol. 2008 Jul-Aug;29(4):233-7. doi: 10.1016/j.amjoto.2007.08.003. Epub 2008 Mar 19.
PURPOSE
Cancerogenesis is a multistage process controlled by many cytokines, including growth factors. The aim of the study was the comparison of transcriptional activity of transforming growth factor beta (TGF-beta) genes in laryngeal squamous cell carcinomas and adjacent nonneoplastic tissues.
MATERIALS AND METHODS
Tissues samples were obtained from 32 patients with laryngeal squamous cell carcinoma in histologic grades G1 to G3 who underwent surgical treatment at the ENT Clinics of Medical University of Silesia in Katowice, Poland. Quantification of gene expression was performed by real-time quantitative reverse transcriptase polymerase chain reaction technique.
RESULTS
In tumor cells, expression of TGF-beta1 and TGF-beta2 isoforms (P < .001) was higher than in normal tissues. There was a positive correlation between the expression of TGF-beta1 and TGF-beta2 genes in tumors (R = 0.78, P = .0000) and adjacent normal tissues (R = 0.77, P = .0000).
CONCLUSIONS
The results suggest that TGF-beta1 and TGF-beta2 messenger RNAs may be useful as molecular markers in distinguishing cancer from nonneoplastic tissues in laryngeal area.
目的
癌症发生是一个由多种细胞因子（包括生长因子）控制的多阶段过程。本研究的目的是比较转化生长因子β（TGF-β）基因在喉鳞状细胞癌和相邻非肿瘤组织中的转录活性。
材料与方法
组织样本取自32例组织学分级为G1至G3的喉鳞状细胞癌患者，这些患者在波兰卡托维兹西里西亚医科大学耳鼻喉科诊所接受了手术治疗。基因表达的定量通过实时定量逆转录聚合酶链反应技术进行。
结果
在肿瘤细胞中，TGF-β1和TGF-β2亚型的表达（P <.001）高于正常组织。肿瘤组织（R = 0.78，P =.0000）和相邻正常组织（R = 0.77，P =.0000）中TGF-β1和TGF-β2基因的表达之间存在正相关。
结论
结果表明，TGF-β1和TGF-β2信使核糖核酸可能作为区分喉区癌组织与非肿瘤组织的分子标志物。
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