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柿(Diospyros kaki)果实中单核细胞增生李斯特菌的发生率及生长情况。

Incidence and growth of Listeria monocytogenes in persimmon (Diospyros kaki) fruit.

作者信息

Uchima C A, de Castro M F P M, Gallo C R, Rezende A C B, Benato E R, Penteado A L

机构信息

Grupo Especial de Engenharia e Pós-Colheita, Instituto de Tecnologia de Alimentos (ITAL), Av. Brasil 2880, Jardim Brasil, Campinas- - S. P., CEP: 13070-178, CP 139, Brazil.

出版信息

Int J Food Microbiol. 2008 Aug 15;126(1-2):235-9. doi: 10.1016/j.ijfoodmicro.2008.05.033. Epub 2008 Jul 1.

Abstract

The incidence of Listeria monocytogenes on persimmon (Diospyros kaki) surface of 'Fuyu' and 'Rama Forte' was evaluated during a 5-month-period (from March to July) of two seasons periods (years 2005 and 2006). The fruits were collected in wholesale and street markets and retail in Sao Paulo and Campinas City, Brazil. A total of 582 fruits were analyzed using the Bax System which is based on the Polymerase Chain Reaction. The ability of this pathogen to grow on the peel and pulp of the two persimmon varieties was also verified at different incubation periods at the temperatures of 10, 20 and 30 degrees C. The growth parameters were obtained by modeling the experimental data using the Gompertz function. The incidence survey showed the absence of L. monocytogenes. The growth curves showed that L. monocytogenes can grow on the peel as well as in the pulp of the two persimmon varieties studied incubated at 10, 20 and 30 degrees C and that low temperatures can reduce the generation rate but does not inhibit its growth.

摘要

在两个季节(2005年和2006年)为期5个月(从3月至7月)的时间里,对‘富有’和‘罗玛·福特’柿子(柿属)表面的单核细胞增生李斯特菌发生率进行了评估。果实采自巴西圣保罗和坎皮纳斯市的批发市场、街边市场及零售店。总共582个果实采用基于聚合酶链反应的Bax系统进行了分析。还在10、20和30摄氏度的不同培养期验证了这种病原菌在这两个柿子品种的果皮和果肉上生长的能力。通过使用Gompertz函数对实验数据进行建模获得了生长参数。发生率调查显示未发现单核细胞增生李斯特菌。生长曲线表明,单核细胞增生李斯特菌能够在10、20和30摄氏度下培养的两个研究柿子品种的果皮和果肉上生长,并且低温可降低生长速率,但不会抑制其生长。

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