Heo C C, Mohamad A M, John J, Baharudin O
Department of Parasitology & Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
Trop Biomed. 2008 Apr;25(1):23-9.
This entomological study was conducted in a man-made freshwater pond in a palm oil plantation in Tanjung Sepat, Selangor from 23 July 2007 by using pig (Sus scrofa) as a carcass model. A 1.5 month old piglet (5 kg), which died of asphyxia after being accidentally crushed by its mother, was thrown into a pond. Observation was made for ten days; one visit per day and climatological data were recorded. On the first two days, the piglet carcass sunk to the bottom of the pond. The carcass floated to the surface on the third day but no fly activities were seen. The blow fly, Chrysomya megacephala and Chrysomya rufifacies started to oviposit on the fourth day. Other than adult flies, a spider (Arachnida) was also observed on the carcass. Bubbles accumulated at the mouthpart, and the abdomen was greenish black. A lot of blow fly eggs were seen on the body surface on the fifth day (floating decay), along with first and second instars C. megacephala crawling under the piglet's skin. On the sixth day, adult blow fly, C. megacephala,and C. rufifacies,and muscid flies, Ophyra spinigera and Musca domestica were observed on to the carcass. High numbers of first and second instars of flies were observed wandering around the body surface with C. megacephala larvae being the predominant species. Two prominent maggot masses occurred on seventh and eighth days. Bloated deterioration stage began on day eighth exposing rib bones, humerus bones and intestines. Carcass was partially sinking and the maggot masses were at the water level. On day ninth, the carcass was partially sinking and three maggot masses were observed on the exposed surface. There were very few adult flies, including a scarab beetle was sighted on the carcass at this stage. The carcass along with the maggots sunk on day tenth, leaving an oily layer on the water surface.
2007年7月23日起，在雪兰莪州丹绒士拔的一个棕榈油种植园内的人工淡水池塘开展了此项昆虫学研究，以猪（野猪）作为尸体模型。一头1.5月龄、体重5千克的仔猪，在被母猪意外压死后窒息死亡，被扔进了池塘。观察持续了十天，每天探访一次并记录气候数据。头两天，仔猪尸体沉到了池塘底部。第三天尸体浮到了水面，但未见苍蝇活动。大头金蝇和红金蝇在第四天开始产卵。除了成年苍蝇，还在尸体上观察到了一只蜘蛛（蛛形纲）。口部积聚了气泡，腹部呈绿黑色。第五天，在尸体表面可见大量的大头金蝇卵（漂浮腐烂期），同时还有一龄和二龄的大头金蝇幼虫在仔猪皮下爬行。第六天，在尸体上观察到了成年大头金蝇、红金蝇以及蝇科的刺足污蝇和家蝇。观察到大量一龄和二龄的蝇幼虫在尸体表面四处爬行，其中大头金蝇幼虫占主导。在第七天和第八天出现了两大团显著的蛆群。第八天开始进入肿胀腐败阶段，肋骨、肱骨和肠道暴露出来。尸体部分下沉，蛆群处于水面位置。第九天，尸体部分下沉，在暴露的表面观察到三团蛆群。此时在尸体上可见的成年苍蝇极少，还发现了一只金龟子。第十天，尸体连同蛆一起下沉，水面上留下了一层油膜。
