4-吡啶酮-3-甲酰胺-1-β-D-核糖核苷三磷酸及其核苷前体在红细胞中的代谢
Metabolism of 4-pyridone-3-carboxamide-1-beta-D-ribonucleoside triphosphate and its nucleoside precursor in the erythrocytes.
作者信息
Slominska E M, Orlewska C, Yuen A, Osman L, Romaszko P, Sokolowska E, Foks H, Simmonds H A, Yacoub M H, Smolenski R T
机构信息
Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2008 Jun;27(6):830-4. doi: 10.1080/15257770802146452.
We recently discovered new nucleotides (4-pyridone-3-carboxamide-1-beta -D-ribonucleoside phosphates) in human erythrocytes. To establish the precursor compound and pathways of nucleotide derivative formation and breakdown, human erythrocytes were incubated for 3 hours with 0.3 mM 4-pyridone-3-carboxamide-1-beta-D-ribonucleoside (4PYR) and erythrocyte concentrations of 4PYR and adenine nucleotides were followed. 4PYR triphosphate increased from 16.1 +/- 0.6 micro M to 74.9 +/- 9.17 and 4PYR monophosphate increased from 5 micro M to 254.7 +/- 13.9 micro M. Conversely, incubation with 0.3 mM 4-pyridone-3-carboxamide (4PY) did not lead to additional 4PYR nucleotide formation. 4PYR nucleotides were catabolized to 4PYR. We conclude that 4PYR nucleotides are formed in erythrocytes by nucleoside kinase-mediated 4PYR phosphorylation and catabolized by 5'nucleotidase-mediated dephosphorylation.
我们最近在人类红细胞中发现了新的核苷酸（4-吡啶酮-3-甲酰胺-1-β-D-核糖核苷磷酸）。为了确定核苷酸衍生物形成和分解的前体化合物及途径，将人类红细胞与0.3 mM 4-吡啶酮-3-甲酰胺-1-β-D-核糖核苷（4PYR）孵育3小时，并跟踪红细胞中4PYR和腺嘌呤核苷酸的浓度。4PYR三磷酸从16.1±0.6 μM增加到74.9±9.17，4PYR单磷酸从5 μM增加到254.7±13.9 μM。相反，与0.3 mM 4-吡啶酮-3-甲酰胺（4PY）孵育不会导致额外的4PYR核苷酸形成。4PYR核苷酸被分解代谢为4PYR。我们得出结论，4PYR核苷酸在红细胞中通过核苷激酶介导的4PYR磷酸化形成，并通过5'-核苷酸酶介导的去磷酸化进行分解代谢。
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