用于依赖贴壁生长动物细胞培养的纤维床生物反应器：第二部分。放大潜力。

A fiber-bed bioreactor for anchorage-dependent animal cell cultures: part II. Scaleup potential.

作者信息

Murakami S, Chiou T W, Wang D I

机构信息

Biotechnology Process Engineering Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

出版信息

Biotechnol Bioeng. 1991 Apr 5;37(8):762-9. doi: 10.1002/bit.260370811.

DOI:10.1002/bit.260370811

PMID:18600673

Abstract

A simple hydrodynamic model is introduced to describe the airlift fiber-bed bioreactor, which can enhance the volumetric productivity of anchorage-dependent animal cell cultures. By applying the model, liquid flow rates and volumetric mass transfer coefficients are predicted and are in agreement with experimental measurements. Consequently, the optimal reactor configuration giving the maximal oxygen supply is derived. Also, theoretical scaleup potential of this concentric internal loop reactor is considered for volumes ranging from 10 to 67,000 L with which cell densities of 5.1 x 10(7) and 1.2 x 10(7) cells/cm(3), respectively, can be maintained.

摘要

引入了一个简单的流体动力学模型来描述气升式纤维床生物反应器，该反应器可提高贴壁依赖型动物细胞培养的体积生产率。通过应用该模型，预测了液体流速和体积传质系数，且与实验测量结果相符。因此，得出了能提供最大氧气供应的最佳反应器配置。此外，还考虑了这种同心内环式反应器在10至67000升体积范围内的理论放大潜力，使用该放大潜力可分别维持5.1×10⁷和1.2×10⁷个细胞/立方厘米的细胞密度。