包埋在气溶胶 OT 反胶束中的酵母醇脱氢酶（YADH）的活性和稳定性。

Activity and stability of yeast alcohol dehydrogenase (YADH) entrapped in aerosol OT reverse micelles.

机构信息

Department of Chemistry, University of Delhi, Delhi, India.

出版信息

Biotechnol Bioeng. 1992 Feb 20;39(4):474-8. doi: 10.1002/bit.260390416.

Abstract

The activity and stability of yeast alcohol dehydrogenase (YADH) entrapped in aerosol OT reverse micellar droplets have been investigated spectrophotometrically. Various physical parameters, e.g., water pool size, w(0), pH, and temperature, were optimized for YADH in water/AOT/isooctane reverse micelles. It was found that the enzyme exhibits maximum activity at w(0) = 28 and pH 8.1. It was more active in reverse micelles than in aqueous buffers at a particular temperature and was denatured at about 307 degrees C in both the systems. At a particular temperature YADH entrapped in reverse micelles was less stable than when it was dissolved in aqueous buffer.

摘要

用分光光度法研究了包埋在气溶胶 OT 反胶束液滴中的酵母醇脱氢酶（YADH）的活性和稳定性。优化了水/AOT/异辛烷反胶束中 YADH 的各种物理参数，例如水池大小 w(0)、pH 值和温度。结果发现，该酶在 w(0)=28 和 pH 8.1 时表现出最大的活性。在特定温度下，它在反胶束中的活性高于在水缓冲液中的活性，并且在两种系统中都在约 307 摄氏度下变性。在特定温度下，包埋在反胶束中的 YADH 不如溶解在水缓冲液中的稳定。