Department of Physiology and Biophysics, Cornell University Medical College, New York, NY 10021, USA.
Biotechnol Bioeng. 1992 Jun 5;40(1):1-7. doi: 10.1002/bit.260400102.
Solvent polarity and pH in the coexisting aqueous phases of aqueous dextran-poly(ethylene glycol) and dextran-Ficoll two-phase systems of varied polymer concentrations were examined using the solvatochromic technique and potentiometric measurements, respectively. The relative solvent polarity of the phases, as measured by the solvatochromic technique, is suggested as a measure of the hydration power of water in the phases of aqueous polymer systems. Partitioning of a series of sulphonephthalein dyes in aqueous dextran-poly(ethylene glycol) and dextran-Ficoll two-phase systems of fixed polymer composition containing 0.01 mol/L universal buffer, pH 7.15, was studied. The results obtained are discussed together with those reported earlier on the physico-chemical features of aqueous media in the coexisting phases of the systems. It is suggested that the two phases of aqueous polymer systems should be viewed as two immiscible water-like solvents. The implications of the suggestion for the theoretical treatment of aqueous polymer two-phase systems are discussed.
使用溶剂极性探针法和电位测定法分别考察了在不同聚合物浓度的水右旋糖酐-聚乙二醇和右旋糖酐-菲可两相间共存的水相中的溶剂极性和 pH 值。通过溶剂极性探针法测量的相的相对溶剂极性被建议作为衡量水在水相聚合物系统中水合能力的指标。在含有 0.01mol/L 通用缓冲液、pH7.15 的固定聚合物组成的水右旋糖酐-聚乙二醇和右旋糖酐-菲可两相间分配一系列亚磺基酞菁染料的实验。将获得的结果与先前报告的系统共存相中水介质的物理化学特性的结果一起进行了讨论。建议将聚合物系统的两相视为两种不混溶的类水溶剂。讨论了该建议对水相聚合物两相系统理论处理的影响。
